ArticleMolecular biology reports2012
Molecular characterization and expression analysis of dihydroflavonol 4-reductase (DFR) gene in Saussurea medusa.
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Identification and characterization of DFR gene family and cloning of candidate genes for anthocyanin biosynthesis in pepper (Capsicum annuum L.).BMC plant biology · 2025Article
- Transcriptome and Metabolome Analyses Reveal the Mechanism of Color Differences in Pomegranate (Plants (Basel, Switzerland) · 2025Article
- Review
- Genome-wide identification of grape ANS gene family and expression analysis at different fruit coloration stages.BMC plant biology · 2023Article
- Effects of editing DFR genes on flowers, leaves, and roots of tobacco.BMC plant biology · 2023Article
- Transcriptome Analysis of Key Genes Involved in Color Variation between Blue and White Flowers ofBioMed research international · 2023Article
- Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza.Scientific reports · 2022Article
- Article
- The novel distribution of intracellular and extracellular flavonoids produced byFrontiers in microbiology · 2022Article
- Functional analysis of the dihydroflavonol 4-reductase family ofHorticulture research · 2022Article
- Comparative transcriptome analysis identified important genes and regulatory pathways for flower color variation in Paphiopedilum hirsutissimum.BMC plant biology · 2021Article
- Identification of Differentially Expressed Genes and Pathways Involved in Growth and Development ofFrontiers in plant science · 2021Article
- Article
- The interplay between miR156/SPL13 and DFR/WD40-1 regulate drought tolerance in alfalfa.BMC plant biology · 2019Article
- Characterization of the complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2019Article
- Whole-genome resequencing and transcriptomic analysis of genes regulating anthocyanin biosynthesis in black rice plants.3 Biotech · 2018Article
- Dihydroflavonol 4-Reductase Genes fromFrontiers in plant science · 2017Article
- Article
- Molecular progress in research on fruit astringency.Molecules (Basel, Switzerland) · 2015Review
- Recent advances on the development and regulation of flower color in ornamental plants.Frontiers in plant science · 2015Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dihydroflavonol 4-reductase (DFR), which catalyzes the reduction of dihydroflavonols to leucoanthocyanins, is a key enzyme in the biosynthesis of anthocyanidins, proanthocyanidins, and other flavonoids of importance in plant development and human nutrition. This study isolated a full length cDNA encoding DFR, designated as SmDFR (GenBank Accession No. EF600682), by screening a cDNA library from a red callus line of Saussurea medusa, which is an endangered, traditional Chinese medicinal plant with high pharmacological value. SmDFR was functionally expressed in yeast (Saccharomyces cerevisiae) to confirm that SmDFR can readily reduce dihydroquercetin (DHQ) and dihydrokampferol (DHK), but it could not reduce dihydromyricetin (DHM). The deduced SmDFR structure shared extensive sequence similarity with previously characterized plant DFRs and phylogenetic analysis showed that it belonged to the plant DFR super-family. SmDFR also possessed flavanone 4-reductase (FNR) activity and can catalyze the conversion of eridictyol to luteoforol. Real-time PCR analysis showed that the expression level of SmDFR was higher in flowers compared with both leaves and roots. This work greatly enhances our knowledge of flavonoid biosynthesis in S. medusa and marks a major advance that could facilitate future genetic modification of S. medusa.
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