ArticleRNA (New York, N.Y.)2011
Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels in Arabidopsis.
Article in RNA (New York, N.Y.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Pooled it
- The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway.Methods in molecular biology (Clifton, N.J.) · 2025Article
- TheGenes · 2024Article
- DRB1, DRB2 and DRB4 Are Required for an Appropriate miRNA-Mediated Molecular Response to Osmotic Stress inInternational journal of molecular sciences · 2024Article
- Molecular Manipulation of the miR160/Genes · 2024Article
- Miniature Inverted-Repeat Transposable Elements: Small DNA Transposons That Have Contributed to PlantPlants (Basel, Switzerland) · 2023Review
- Phased small RNA-mediated systemic signaling in plants.Science advances · 2022Article
- Immunocapture of dsRNA-bound proteins provides insight into Tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector.The Plant cell · 2021Article
- MicroRNA-Mediated Responses to Cadmium Stress inPlants (Basel, Switzerland) · 2021Article
- Disruption of miRNA sequences by TALENs and CRISPR/Cas9 induces varied lengths of miRNA production.Plant biotechnology journal · 2020Article
- Double-Stranded-RNA-Binding Protein 2 Participates in Antiviral Defense.Journal of virology · 2020Article
- DRB1, DRB2 and DRB4 Are Required for Appropriate Regulation of the microRNA399/Plants (Basel, Switzerland) · 2019Article
- Review
- CRISPR/Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max and Medicago truncatula.Plant biotechnology journal · 2018Article
- New DRB complexes for new DRB functions in plants.RNA biology · 2017Article
- Arabidopsis proteins with a transposon-related domain act in gene silencing.Nature communications · 2017Article
- A complex ofRNA (New York, N.Y.) · 2017Article
- A specific dsRNA-binding protein complex selectively sequesters endogenous inverted-repeat siRNA precursors and inhibits their processing.Nucleic acids research · 2017Article
- Double-stranded RNA-binding protein DRB3 negatively regulates anthocyanin biosynthesis by modulating PAP1 expression in Arabidopsis thaliana.Journal of plant research · 2017Article
- DNA Methylation Influences the Expression of DICER-LIKE4 Isoforms, Which Encode Proteins of Alternative Localization and Function.The Plant cell · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biogenesis of the vast majority of plant siRNAs depends on the activity of the plant-specific RNA polymerase IV (PolIV) enzyme. As part of the RNA-dependent DNA methylation (RdDM) process, PolIV-dependent siRNAs (p4-siRNAs) are loaded onto an ARGONAUTE4-containing complex and guide de novo DNA methyltransferases to target loci. Here we show that the double-stranded RNA binding proteins DRB2 and DRB4 are required for proper accumulation of p4-siRNAs. In flowers, loss of DRB2 results in increased accumulation of p4-siRNAs but not ta-siRNAs, inverted repeat (IR)-derived siRNAs, or miRNA. Loss of DRB2 does not impair uniparental expression of p4-dependent siRNAs in developing endosperm, indicating that p4-siRNA increased accumulation is not the result of the activation of the polIV pathway in the male gametophyte. In contrast to drb2, drb4 mutants exhibit reduced p4-siRNA levels, but the extent of this reduction is variable, according to the nature and size of the p4-siRNAs. Loss of DRB4 also leads to a spectacular increase of p4-independent IR-derived 24-nt siRNAs, suggesting a reallocation of factors from p4-dependent to p4-independent siRNA pathways in drb4. Opposite effects of drb2 and drb4 mutations on the accumulation of p4-siRNAs were also observed in vegetative tissues. Moreover, transgenic plants overexpressing DRB2 mimicked drb4 mutants at the morphological and molecular levels, confirming the antagonistic roles of DRB2 and DRB4.
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Registered trials
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