ArticleVirology2011
Use of a tandem affinity purification assay to detect interactions between West Nile and dengue viral proteins and proteins of the mosquito vector.
Article in Virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Biological determinants perpetuating the transmission dynamics of mosquito-borne flaviviruses.Emerging microbes & infections · 2023Review
- Review
- Assessing Aedes aegypti candidate genes during viral infection and Wolbachia-mediated pathogen blocking.Insect molecular biology · 2022Article
- Revisiting dengue virus-mosquito interactions: molecular insights into viral fitness.The Journal of general virology · 2021Article
- Evaluation of Total Female and MaleInsects · 2021Article
- Review
- Aedes aegypti SNAP and a calcium transporter ATPase influence dengue virus dissemination.PLoS neglected tropical diseases · 2021Article
- Article
- Selection on Aedes aegypti alters Wolbachia-mediated dengue virus blocking and fitness.Nature microbiology · 2019Article
- Discovery of Novel Thrips Vector Proteins That Bind to the Viral Attachment Protein of the Plant Bunyavirus Tomato Spotted Wilt Virus.Journal of virology · 2019Article
- Inhibition of dengue virus by curcuminoids.Antiviral research · 2019Article
- Elucidation of host-pathogen protein-protein interactions to uncover mechanisms of host cell rewiring.Current opinion in microbiology · 2017Review
- DenvInt: A database of protein-protein interactions between dengue virus and its hosts.PLoS neglected tropical diseases · 2017Article
- A relevant in vitro human model for the study of Zika virus antibody-dependent enhancement.The Journal of general virology · 2017Article
- A novel mosquito ubiquitin targets viral envelope protein for degradation and reduces virion production during dengue virus infection.Biochimica et biophysica acta · 2016Article
- Dengue Virus Infection of Aedes aegypti Requires a Putative Cysteine Rich Venom Protein.PLoS pathogens · 2015Article
- A basic cluster in the N terminus of yellow fever virus NS2A contributes to infectious particle production.Journal of virology · 2015Article
- Interaction between Flavivirus and Cytoskeleton during Virus Replication.BioMed research international · 2015Review
- Quantitative proteomic analysis of host-virus interactions reveals a role for Golgi brefeldin A resistance factor 1 (GBF1) in dengue infection.Molecular & cellular proteomics : MCP · 2014Article
- Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
West Nile and dengue viruses are (re)emerging mosquito-borne flaviviruses that cause significant morbidity and mortality in man. The identification of mosquito proteins that associate with flaviviruses may provide novel targets to inhibit infection of the vector or block transmission to humans. Here, a tandem affinity purification (TAP) assay was used to identify 18 mosquito proteins that interact with dengue and West Nile capsid, envelope, NS2A or NS2B proteins. We further analyzed the interaction of mosquito cadherin with dengue and West Nile virus envelope protein using co-immunoprecipitation and immunofluorescence. Blocking the function of select mosquito factors, including actin, myosin, PI3-kinase and myosin light chain kinase, reduced both dengue and West Nile virus infection in mosquito cells. We show that the TAP method may be used in insect cells to accurately identify flaviviral-host protein interactions. Our data also provides several targets for interrupting flavivirus infection in mosquito vectors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.