ArticleInsect molecular biology2011
Comparison of transgene expression in Aedes aegypti generated by mariner Mos1 transposition and ΦC31 site-directed recombination.
Article in Insect molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
- Engineered promoter system enables high-efficiency transgenic CRISPR editing in Malaria transmitting mosquitoZoological research · 2026Article
- Transgene-induced cell death following dengue-2 virus infection in Aedes aegypti.Scientific reports · 2023Article
- Optimizing CRE and PhiC31 mediated recombination inFrontiers in bioengineering and biotechnology · 2023Article
- The Aedes aegypti siRNA pathway mediates broad-spectrum defense against human pathogenic viruses and modulates antibacterial and antifungal defenses.PLoS biology · 2022Article
- The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in TransgenicViruses · 2020Article
- Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila.Insect biochemistry and molecular biology · 2020Article
- Engineered resistance to Zika virus in transgenicProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- Cre/lox-Recombinase-Mediated Cassette Exchange for Reversible Site-Specific Genomic Targeting of the Disease Vector, Aedes aegypti.Scientific reports · 2017Article
- Tools for Anopheles gambiae Transgenesis.G3 (Bethesda, Md.) · 2015Article
- Disruption of dengue virus transmission by mosquitoes.Current opinion in insect science · 2015Article
- Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes.Insects · 2015Review
- Heritable CRISPR/Cas9-mediated genome editing in the yellow fever mosquito, Aedes aegypti.PloS one · 2015Article
- Regulation of the gut-specific carboxypeptidase: a study using the binary Gal4/UAS system in the mosquito Aedes aegypti.Insect biochemistry and molecular biology · 2014Article
- Efficient ΦC31 integrase-mediated site-specific germline transformation of Anopheles gambiae.Nature protocols · 2014Article
- Fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse Aedes aegypti strain.PLoS neglected tropical diseases · 2014Article
- Novel Genetic and Molecular Tools for the Investigation and Control of Dengue Virus Transmission by Mosquitoes.Current tropical medicine reports · 2014Article
- Juvenile Hormone Biosynthesis in Insects: What Is New, What Do We Know, and What Questions Remain?International scholarly research notices · 2014Review
- Towards the elements of successful insect RNAi.Journal of insect physiology · 2013Review
- Isolation of midgut escape mutants of two American genotype dengue 2 viruses from Aedes aegypti.Virology journal · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Transgenic mosquitoes generated by transposable elements (TEs) often poorly express transgenes owing to position effects. To avoid these effects, the ΦC31 site-directed recombination system was used to insert transgenes into a locus favourable for gene expression in Aedes aegypti. We describe phenotypes of mariner Mos1 TE and ΦC31 transgenic mosquitoes expressing the enhanced green fluorescent protein (EGFP) reporter in midguts of blood-fed females. Mosquitoes of nine TE-generated lines [estimated transformation frequency (TF): 9.3%] clearly expressed the eye-specific selection marker but only 2/9 lines robustly expressed the EGFP reporter. The piggyBac TE-generated ΦC31 docking strain, attP26, supported recombination with attB site containing donors at an estimated TF of 1.7-4.9%. Using a codon-optimized ΦC31 integrase mutant instead of the 'wild-type' enzyme did not affect TF. Site-directed recombination of line attP26 with an attB-containing donor expressing EGFP from the Ae. aegypti carboxypeptidase promoter produced one transgenic line with blood-fed females expressing the reporter in midgut tissue. Docking strain attP26 also supported robust expression of Flock House virus B2 from the Ae. aegypti polyubiquitin promoter. Our data confirm that eye-specific selection marker expression alone is not a reliable indicator for robust gene-of-interest expression in Ae. aegypti and that the ΦC31 system can ensure predictable transgene expression in this mosquito species.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.