ArticleJournal of biomolecular NMR2011
Overexpression of a homogeneous oligosaccharide with 13C labeling by genetically engineered yeast strain.
Article in Journal of biomolecular NMR, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Review
- Primary Structure of Glycans by NMR Spectroscopy.Chemical reviews · 2023Review
- On the use of Pichia pastoris for isotopic labeling of human GPCRs for NMR studies.Journal of biomolecular NMR · 2018Review
- Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems.Journal of biomolecular NMR · 2018Article
- Chemical and Biophysical Approaches for Complete Characterization of Lectin-Carbohydrate Interactions.Methods in enzymology · 2018Article
- Exploiting UniformlyJournal of the American Chemical Society · 2017Article
- Insights from NMR Spectroscopy into the Conformational Properties of Man-9 and Its Recognition by Two HIV Binding Proteins.Chembiochem : a European journal of chemical biology · 2017Article
- Paramagnetic NMR probes for characterization of the dynamic conformations and interactions of oligosaccharides.Glycoconjugate journal · 2015Review
- NMR structure analysis of uniformly 13C-labeled carbohydrates.Journal of biomolecular NMR · 2014Article
- Article
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Authors and funding
5 authors.
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Abstract
This report describes a novel method for overexpression of (13)C-labeled oligosaccharides using genetically engineered Saccharomyces cerevisiae cells, in which a homogeneous high-mannose-type oligosaccharide accumulates because of deletions of genes encoding three enzymes involved in the processing pathway of asparagine-linked oligosaccharides in the Golgi complex. Using uniformly (13)C-labeled glucose as the sole carbon source in the culture medium of these engineered yeast cells, high yields of the isotopically labeled Man(8)GlcNAc(2) oligosaccharide could be successfully harvested from glycoprotein extracts of the cells. Furthermore, (13)C labeling at selected positions of the sugar residues in the oligosaccharide could be achieved using a site-specific (13)C-enriched glucose as the metabolic precursor, facilitating NMR spectral assignments. The (13)C-labeling method presented provides the technical basis for NMR analyses of structures, dynamics, and interactions of larger, branched oligosaccharides.
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