ArticleMolecular genetics and genomics : MGG2011
Identification of genes necessary for wild-type levels of seed phytic acid in Arabidopsis thaliana using a reverse genetics approach.
Article in Molecular genetics and genomics : MGG, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 71 citations in OpenAlex.
- Review
- Climate change and the antinutrient-antioxidant puzzle in common bean seeds.Journal of the science of food and agriculture · 2026Review
- A minimum catalytic unit for synthesis of InsP6 and 5-PP-InsP5 in Arabidopsis.The Biochemical journal · 2025Article
- Conservation of heat stress acclimation by the IPK2-type kinases that control the synthesis of the inositol pyrophosphate 4/6-InsP7 in land plants.PLoS genetics · 2025Article
- OsIPK1 frameshift mutations disturb phosphorus homeostasis and impair starch synthesis during grain filling in rice.Plant molecular biology · 2024Article
- A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation.Nature communications · 2024Article
- An Overview of Targeted Genome Editing Strategies for Reducing the Biosynthesis of Phytic Acid: an Anti-nutrient in Crop Plants.Molecular biotechnology · 2024Review
- Diversification in the inositol tris/tetrakisphosphate kinase (ITPK) family: crystal structure and enzymology of the outlier AtITPK4.The Biochemical journal · 2023Article
- Plant phosphate nutrition: sensing the stress.Stress biology · 2022Review
- Arabidopsis inositol polyphosphate kinases IPK1 and ITPK1 modulate crosstalk between SA-dependent immunity and phosphate-starvation responses.Plant cell reports · 2022Article
- Genetic manipulation of anti-nutritional factors in major crops for a sustainable diet in future.Frontiers in plant science · 2022Review
- Network Inference of Transcriptional Regulation in Germinating Low Phytic Acid Soybean Seeds.Frontiers in plant science · 2021Article
- Gene editing of three BnITPK genes in tetraploid oilseed rape leads to significant reduction of phytic acid in seeds.Plant biotechnology journal · 2020Article
- An ATP-responsive metabolic cassette comprised of inositol tris/tetrakisphosphate kinase 1 (ITPK1) and inositol pentakisphosphate 2-kinase (IPK1) buffers diphosphosphoinositol phosphate levels.The Biochemical journal · 2020Article
- Inositol Pyrophosphate Pathways and Mechanisms: What Can We Learn from Plants?Molecules (Basel, Switzerland) · 2020Review
- Inositol hexakisphosphate biosynthesis underpins PAMP-triggered immunity to Pseudomonas syringae pv. tomato in Arabidopsis thaliana but is dispensable for establishment of systemic acquired resistance.Molecular plant pathology · 2020Article
- Allelic Variants of CRISPR/Cas9 Induced Mutation in an Inositol Trisphosphate 5/6 Kinase Gene Manifest Different Phenotypes in Barley.Plants (Basel, Switzerland) · 2020Article
- Article
- Can Inositol Pyrophosphates Inform Strategies for Developing Low Phytate Crops?Plants (Basel, Switzerland) · 2020Review
- Knockout ofFrontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The majority of phosphorus (P) in seeds is found in phytic acid (InsP(6)) which accumulates as the mixed salt phytate. InsP(6) is generally considered to be an anti-nutrient and the development of low phytic acid (lpa) seed crops is of significant interest. We have employed a reverse genetics approach to examine the impact of disrupting genes involved in inositol phosphate metabolism on Arabidopsis seed InsP(6) levels. Our analysis revealed that knockout mutations in three genes (AtITPK1, AtITPK4, and AtMIK/At5g58730) reduced seed InsP(6) in addition to knockouts of four previously reported genes (AtIPK1, AtIPK2β, AtMRP5, and At5g60760). Seeds of these lpa mutants also exhibited reduced germination under various stress conditions. The greatest reduction in InsP(6) (>70%) was observed in atmrp5 seeds which were also among the least sensitive to the stresses examined. Expression analysis of the lpa genes revealed three distinct patterns in developing siliques consistent with their presumed roles. Disruption of each lpa gene resulted in changes in the expression in some of the other lpa genes indicating that transcription of lpa genes is modulated by other constituents of InsP(6) metabolism. While all the lpa genes represent possible targets for genetic engineering of low phytate seed crops, mutations in AtMRP5, AtMIK, and At5g60760 may be most successful for conventional approaches such as mutation breeding.
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