Evidence map›Paper›PMID 21698257›Full record

ArticlePloS one2011

SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor.

Marco A R Vinolo, G John Ferguson, Suhasini Kulkarni, George Damoulakis, Karen Anderson, Mohammad Bohlooly-Y, Len Stephens, Phillip T Hawkins, Rui Curi

Registry-linked trialOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02699905 (Neutrophil FFA2/GPR43 Receptor Expression in Patients With the Diagnosis of Sepsis), which is not on this map. Cited by 141 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
141citing papers in PubMed, 3 pooled it
9.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02699905 withdrawnnot on this mapstarted 2016, after this paper: background citation

Neutrophil FFA2/GPR43 Receptor Expression in Patients With the Diagnosis of Sepsis

TypeobservationalSponsorMilton S. Hershey Medical CenterRan2016 to 2017Enrolled0ConditionsSepsis, Septic Shock, Multiple Organ Dysfunction, Intra-abdominal SepsisArmsphlebotomy
3 · Its place in the literature

Who cites it

141 citing papers in PubMed, 3 syntheses or guidelines pooled it, 282 citations in OpenAlex.

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81 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Marco A R VinoloDepartment of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
G John Ferguson
Suhasini Kulkarni
George Damoulakis
Karen Anderson
Mohammad Bohlooly-Y
Len Stephens
Phillip T Hawkins
Rui Curi
Babraham Institute · GBUniversidade de São Paulo · BRAstraZeneca (Sweden) · SE

Funding

Biotechnology and Biological Sciences Research Council BBS/E/B/0000C218
6 · The paper itself

Abstract

Short chain fatty acids (SCFAs) have recently attracted attention as potential mediators of the effects of gut microbiota on intestinal inflammation. Some of these effects have been suggested to occur through the direct actions of SCFAs on the GPR43 receptor in neutrophils, though the precise role of this receptor in neutrophil activation is still unclear. We show that mouse bone marrow derived neutrophils (BMNs) can chemotax effectively through polycarbonate filters towards a source of acetate, propionate or butyrate. Moreover, we show that BMNs move with good speed and directionality towards a source of propionate in an EZ-Taxiscan chamber coated with fibrinogen. These effects of SCFAs were mimicked by low concentrations of the synthetic GPR43 agonist phenylacetamide-1 and were abolished in GPR43(-/-) BMNs. SCFAs and phenylacetamide-1 also elicited GPR43-dependent activation of PKB, p38 and ERK and these responses were sensitive to pertussis toxin, indicating a role for Gi proteins. Phenylacetamide-1 also elicited rapid and transient activation of Rac1/2 GTPases and phosphorylation of ribosomal protein S6. Genetic and pharmacological intervention identified important roles for PI3Kγ, Rac2, p38 and ERK, but not mTOR, in GPR43-dependent chemotaxis. These results identify GPR43 as a bona fide chemotactic receptor for neutrophils in vitro and start to define important elements in its signal transduction pathways.

Indexed as

AnimalsBlotting, WesternChemotaxis, LeukocyteFatty AcidsMiceMice, KnockoutNeutrophilsReceptors, G-Protein-CoupledFatty AcidsFfar2 protein, mouseReceptors, G-Protein-Coupled

Identifiers

PMID21698257
PMCPMC3115979
OpenAlexW2119522619

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.