ArticlePloS one2011
Identification and functional analysis of epigenetically silenced microRNAs in colorectal cancer cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 59 citations in OpenAlex.
- miR-1247 functions by targeting cartilage transcription factor SOX9.The Journal of biological chemistry · 2013Trial
- MicroRNA Methylome Signature and Their Functional Roles in Colorectal Cancer Diagnosis, Prognosis, and Chemoresistance.International journal of molecular sciences · 2022Review
- MiRNA expression profiling and emergence of new prognostic signature for oral squamous cell carcinoma.Scientific reports · 2021Article
- Epigenetically regulated miR-1247 functions as a novel tumour suppressor via MYCBP2 in methylator colon cancers.British journal of cancer · 2018Article
- Epigenetics and MicroRNAs in Cancer.International journal of molecular sciences · 2018Review
- Review
- Up-regulation of CIT promotes the growth of colon cancer cells.Oncotarget · 2017Article
- A sketch of known and novel MYCN-associated miRNA networks in neuroblastoma.Oncology reports · 2017Article
- Epigenetically altered miR-1247 functions as a tumor suppressor in pancreatic cancer.Oncotarget · 2017Article
- Reciprocal regulation between microRNAs and epigenetic machinery in colorectal cancer.Oncology letters · 2017Article
- MicroRNA Expression Profile Reveals miR-17-92 and miR-143-145 Cluster in Synchronous Colorectal Cancer.Medicine · 2015Article
- Interleukin-6 mediated upregulation of CYP1B1 and CYP2E1 in colorectal cancer involves DNA methylation, miR27b and STAT3.British journal of cancer · 2014Article
- Epigenetically regulated MIR941 and MIR1247 target gastric cancer cell growth and migration.Epigenetics · 2014Article
- DNA methylation, microRNAs, and their crosstalk as potential biomarkers in hepatocellular carcinoma.World journal of gastroenterology · 2014Review
- Putting cells to sleep for future science.Nature biotechnology · 2014Article
- Functional screening identifies miRNAs influencing apoptosis and proliferation in colorectal cancer.PloS one · 2014Article
- Epigenetic alteration and microRNA dysregulation in cancer.Frontiers in genetics · 2013Review
- Epigenetics and colorectal cancer pathogenesis.Cancers · 2013Article
- Dysregulation of cholesterol homeostasis in human prostate cancer through loss of ABCA1.Cancer research · 2013Article
- Double restriction-enzyme digestion improves the coverage and accuracy of genome-wide CpG methylation profiling by reduced representation bisulfite sequencing.BMC genomics · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Abnormal microRNA (miRNA) expression has been linked to the development and progression of several human cancers, and such dysregulation can result from aberrant DNA methylation. While a small number of miRNAs is known to be regulated by DNA methylation, we postulated that such epigenetic regulation is more prevalent. By combining MBD-isolated Genome Sequencing (MiGS) to evaluate genome-wide DNA methylation patterns and microarray analysis to determine miRNA expression levels, we systematically searched for candidate miRNAs regulated by DNA methylation in colorectal cancer cell lines. We found 64 miRNAs to be robustly methylated in HCT116 cells; eighteen of them were located in imprinting regions or already reported to be regulated by DNA methylation. For the remaining 46 miRNAs, expression levels of 18 were consistent with their DNA methylation status. Finally, 8 miRNAs were up-regulated by 5-aza-2'-deoxycytidine treatment and identified to be novel miRNAs regulated by DNA methylation. Moreover, we demonstrated the functional relevance of these epigenetically silenced miRNAs by ectopically expressing select candidates, which resulted in inhibition of growth and migration of cancer cells. In addition to reporting these findings, our study also provides a reliable, systematic strategy to identify DNA methylation-regulated miRNAs by combining DNA methylation profiles and expression data.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.