ArticlePloS one2011
Development of an efficient recombinant mosquito densovirus-mediated RNA interference system and its preliminary application in mosquito control.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 83 citations in OpenAlex.
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- RNAi-Mediated Silencing ofInsects · 2024Article
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- Outlook on RNAi-Based Strategies for ControllingPathogens (Basel, Switzerland) · 2023Review
- Engineered Gut Symbiotic Bacterium-Mediated RNAi for Effective Control ofMicrobiology spectrum · 2023Article
- Mosquito densovirus significantly reduces the vector susceptibility to dengue virus serotype 2 in Aedes albopictus mosquitoes (Diptera: Culicidae).Infectious diseases of poverty · 2023Article
- Effect of marker-free transgenic Chlamydomonas on the control of Aedes mosquito population and on plankton.Parasites & vectors · 2023Article
- Development of an RNAi-based microalgal larvicide for the control of Aedes aegypti.Parasites & vectors · 2021Article
- Delivery Methods for RNAi in Mosquito Larvae.Journal of insect science (Online) · 2020Review
- Insecticidal Gene Silencing by RNAi in the Neotropical Region.Neotropical entomology · 2020Review
- Novel RNAi delivery systems in the control of medical and veterinary pests.Current opinion in insect science · 2019Review
- Development of large-scale mosquito densovirus production by in vivo methods.Parasites & vectors · 2019Article
- A Novel Densovirus Isolated From the Asian Tiger Mosquito Displays Varied Pathogenicity Depending on Its Host Species.Frontiers in microbiology · 2019Article
- Densonucleosis viruses ('densoviruses') for mosquito and pathogen control.Current opinion in insect science · 2018Review
- LinearPeerJ · 2018Article
- Engineered Flock House Virus for Targeted Gene Suppression Through RNAi in Fruit Flies (Frontiers in physiology · 2018Article
- Review
- Viral Delivery of dsRNA for Control of Insect Agricultural Pests and Vectors of Human Disease: Prospects and Challenges.Frontiers in physiology · 2017Review
- Article
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Aedes aegypti densovirus (AeDNV) has potential as a delivery vector for foreign nucleic acids into mosquito cells. In this study, we investigated the ability of plasmids containing recombinant viral transducing genome to induce RNA interference (RNAi) effects in C6/C36 cells. We then evaluated the efficiency of a recombinant AeDNV vector to induce RNAi in Aedes albopictus larvae. We found that the expression of V-ATPase was inhibited by up to 90% at 96 h post-transfection in transfected C6/C36 cells. In addition, the bioinsecticidal activities of various RNAi-expressing AeDNV vectors used to infect Ae. albopictus larvae were also tested. We found that when Ae. albopictus larvae were infected with recombinant AeDNV, expression of V-ATPase was downregulated by nearly 70% compared to controls. Furthermore, the median survival time bioassays demonstrated that recombinant AeDNV caused more serious pathogenic effects than the wild type virus. This is the first report showing that recombinant virus plasmid and corresponding recombinant AeDNV can be used as an effective in vitro and in vivo RNAi delivery system, respectively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.