ArticlePloS one2011
Proteomic analysis of the secretory response of Aspergillus niger to D-maltose and D-xylose.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 69 citations in OpenAlex.
- Multi-omics analysis of Trichoderma reesei mutant with high glucanase activity.Scientific reports · 2025Article
- A novel sucrose-inducible expression system and its application for production of biomass-degrading enzymes in Aspergillus niger.Biotechnology for biofuels and bioproducts · 2023Article
- Disease-associated dysbiosis and potential therapeutic role of Akkermansia muciniphila, a mucus degrading bacteria of gut microbiome.Folia microbiologica · 2022Review
- Integration of enzyme constraints in a genome-scale metabolic model of Aspergillus niger improves phenotype predictions.Microbial cell factories · 2021Article
- Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species.Scientific reports · 2018Article
- Protein profile in Aspergillus nidulans recombinant strains overproducing heterologous enzymes.Microbial biotechnology · 2018Article
- A community-driven reconstruction of theFungal biology and biotechnology · 2018Article
- A two-dimensional protein map of Pleurotus ostreatus microsomes-proteome dynamics.Folia microbiologica · 2016Article
- Deciphering the signaling mechanisms of the plant cell wall degradation machinery in Aspergillus oryzae.BMC systems biology · 2015Article
- Spatial differentiation of gene expression in Aspergillus niger colony grown for sugar beet pulp utilization.Scientific reports · 2015Article
- Aspergillus niger membrane-associated proteome analysis for the identification of glucose transporters.Biotechnology for biofuels · 2015Article
- Comparative Secretome Analysis of Trichoderma reesei and Aspergillus niger during Growth on Sugarcane Biomass.PloS one · 2015Article
- The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation.Fungal genetics and biology : FG & B · 2014Article
- A genomic survey of proteases in Aspergilli.BMC genomics · 2014Article
- Characterization of Alternaria infectoria extracellular vesicles.Medical mycology · 2014Article
- The transcriptomic fingerprint of glucoamylase over-expression in Aspergillus niger.BMC genomics · 2012Article
- d-Xylose concentration-dependent hydrolase expression profiles and the function of CreA and XlnR in Aspergillus niger.Applied and environmental microbiology · 2012Article
- The Penicillium echinulatum secretome on sugar cane bagasse.PloS one · 2012Article
- Article
- Biochemical and molecular characterization of secreted α-xylosidase from Aspergillus niger.The Journal of biological chemistry · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungi utilize polysaccharide substrates through extracellular digestion catalyzed by secreted enzymes. Thus far, protein secretion by the filamentous fungus Aspergillus niger has mainly been studied at the level of individual proteins and by genome and transcriptome analyses. To extend these studies, a complementary proteomics approach was applied with the aim to investigate the changes in secretome and microsomal protein composition resulting from a shift to a high level secretion condition. During growth of A. niger on D-sorbitol, small amounts of D-maltose or D-xylose were used as inducers of the extracellular amylolytic and xylanolytic enzymes. Upon induction, protein compositions in the extracellular broth as well as in enriched secretory organelle (microsomal) fractions were analyzed using a shotgun proteomics approach. In total 102 secreted proteins and 1,126 microsomal proteins were identified in this study. Induction by D-maltose or D-xylose resulted in the increase in specific extracellular enzymes, such as glucoamylase A on D-maltose and β-xylosidase D on D-xylose, as well as of microsomal proteins. This reflects the differential expression of selected genes coding for dedicated extracellular enzymes. As expected, the addition of extra D-sorbitol had no effect on the expression of carbohydrate-active enzymes, compared to addition of D-xylose or D-maltose. Furthermore, D-maltose induction caused an increase in microsomal proteins related to translation (e.g., Rpl15) and vesicular transport (e.g., the endosomal-cargo receptor Erv14). Millimolar amounts of the inducers D-maltose and D-xylose are sufficient to cause a direct response in specific protein expression levels. Also, after induction by D-maltose or D-xylose, the induced enzymes were found in microsomes and extracellular. In agreement with our previous findings for D-xylose induction, D-maltose induction leads to recruitment of proteins involved in proteasome-mediated degradation.
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