ArticleBiomedical optics express2011
Integrated holographic system for all-optical manipulation of developing embryos.
Article in Biomedical optics express, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 31 citations in OpenAlex.
- Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond.ACS photonics · 2024Review
- Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage.Journal of assisted reproduction and genetics · 2021Article
- Femtosecond laser is effective tool for zona pellucida engraving and tagging of preimplantation mammalian embryos.Journal of assisted reproduction and genetics · 2019Article
- Application of femtosecond laser microsurgery in assisted reproductive technologies for preimplantation embryo tagging.Biomedical optics express · 2019Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Fast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiation.Scientific reports · 2013Article
- Femtosecond optical transfection of individual mammalian cells.Nature protocols · 2013Article
- Cell signaling experiments driven by optical manipulation.International journal of molecular sciences · 2013Review
- Femtosecond optoinjection of intact tobacco BY-2 cells using a reconfigurable photoporation platform.PloS one · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate a system for the combined optical injection and trapping of developing embryos. A Ti:sapphire femtosecond laser in tandem with a spatial light modulator, is used to perform fast and accurate beam-steering and multiplexing. We show successful intracellular delivery of a range of impermeable molecules into individual blastomeres of the annelid Pomatoceros lamarckii embryo by optoinjection, even when the embryo is still enclosed in a chorion. We also demonstrate the ability of the femtosecond laser optoinjection to deliver materials into inner layers of cells in a well-developed embryo. By switching to the continuous wave mode of the Ti:sapphire laser, the same system can be employed to optically trap and orient the 60 μm sized P. lamarckii embryo whilst maintaining its viability. Hence, a complete all-optical manipulation platform is demonstrated paving the way towards single-cell genetic modification and cell lineage mapping in emerging developmental biology model species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.