ArticleBiomedical optics express2011
Two-photon excited autofluorescence imaging of freshly isolated frog retinas.
Article in Biomedical optics express, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Overcoming size barriers in retinal gene therapy via lipid nanoparticle-mediated delivery of full-length eys DNA.Drug delivery and translational research · 2026Article
- Quantification of Photoreceptors' Changes in a Diabetic Retinopathy Model with Two-Photon Imaging Microscopy.International journal of molecular sciences · 2024Article
- Focused Ultrasound as a Novel Non-Invasive Method for the Delivery of Gold Nanoparticles to Retinal Ganglion Cells.Translational vision science & technology · 2024Article
- Interpretation of anatomic correlates of outer retinal bands in optical coherence tomography.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Arrangement of the photoreceptor mosaic in a diabetic rat model imaged with multiphoton microscopy.Biomedical optics express · 2020Article
- In vivo super-resolution retinal imaging through virtually structured detection.Journal of biomedical optics · 2016Article
- Two-Photon Autofluorescence Imaging Reveals Cellular Structures Throughout the Retina of the Living Primate Eye.Investigative ophthalmology & visual science · 2016Article
- Periscope for noninvasive two-photon imaging of murine retina in vivo.Biomedical optics express · 2015Article
- Rapid super-resolution line-scanning microscopy through virtually structured detection.Optics letters · 2015Article
- Super-Resolution Scanning Laser Microscopy Based on Virtually Structured Detection.Critical reviews in biomedical engineering · 2015Review
- Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy.Biomedical optics express · 2014Article
- In vivo two-photon imaging of the mouse retina.Biomedical optics express · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The purpose of this study was to investigate cellular sources of autofluorescence signals in freshly isolated frog (Rana pipiens) retinas. Equipped with an ultrafast laser, a laser scanning two-photon excitation fluorescence microscope was employed for sub-cellular resolution examination of both sliced and flat-mounted retinas. Two-photon imaging of retinal slices revealed autofluorescence signals over multiple functional layers, including the photoreceptor layer (PRL), outer nuclear layer (ONL), outer plexiform layer (OPL), inner nuclear layer (INL), inner plexiform layer (IPL), and ganglion cell layer (GCL). Using flat-mounted retinas, depth-resolved imaging of individual retinal layers further confirmed multiple sources of autofluorescence signals. Cellular structures were clearly observed at the PRL, ONL, INL, and GCL. At the PRL, the autofluorescence was dominantly recorded from the intracellular compartment of the photoreceptors; while mixed intracellular and extracellular autofluorescence signals were observed at the ONL, INL, and GCL. High resolution autofluorescence imaging clearly revealed mosaic organization of rod and cone photoreceptors; and sub-cellular bright autofluorescence spots, which might relate to connecting cilium, was observed in the cone photoreceptors only. Moreover, single-cone and double-cone outer segments could be directly differentiated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.