ArticlePlant physiology2011
Assembly and sorting of the tonoplast potassium channel AtTPK1 and its turnover by internalization into the vacuole.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 43 citations in OpenAlex.
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- Protein Biosynthesis and Maturation in the ER.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Two γ-zeins induce the unfolded protein response.Plant physiology · 2021Article
- New Insights into Plant TPK Ion Channel Evolution.Plants (Basel, Switzerland) · 2021Article
- Screening and identification of salt-tolerance genes in Sophora alopecuroides and functional verification of SaAQP.Planta · 2021Article
- Go your own way: membrane-targeting sequences.Plant physiology · 2021Article
- Vacuolar membrane structures and their roles in plant-pathogen interactions.Plant molecular biology · 2019Review
- A Review of Plant Vacuoles: Formation, Located Proteins, and Functions.Plants (Basel, Switzerland) · 2019Review
- Photosynthesis in Arabidopsis Is Unaffected by the Function of the Vacuolar KPlant physiology · 2019Article
- Tethering of Multi-Vesicular Bodies and the Tonoplast to the Plasma Membrane in Plants.Frontiers in plant science · 2019Article
- Manipulating Endoplasmic Reticulum-Plasma Membrane Tethering in Plants Through Fluorescent Protein Complementation.Frontiers in plant science · 2019Article
- Protein Storage Vacuoles Originate from Remodeled Preexisting Vacuoles inPlant physiology · 2018Article
- The glycan-dependent ERAD machinery degrades topologically diverse misfolded proteins.The Plant journal : for cell and molecular biology · 2018Article
- Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress.BMC plant biology · 2018Article
- Phosphatidylinositol 3-phosphate-binding protein AtPH1 controls the localization of the metal transporter NRAMP1 inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- In-Depth Genomic and Transcriptomic Analysis of Five KFrontiers in plant science · 2017Article
- The Arabidopsis tonoplast is almost devoid of glycoproteins with complex N-glycans, unlike the rat lysosomal membrane.Journal of experimental botany · 2016Article
- K₂p channels in plants and animals.Pflugers Archiv : European journal of physiology · 2015Review
- REGULATOR OF BULB BIOGENESIS1 (RBB1) Is Involved in Vacuole Bulb Formation in Arabidopsis.PloS one · 2015Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly, sorting signals, and turnover of the tonoplast potassium channel AtTPK1 of Arabidopsis (Arabidopsis thaliana) were studied. We used transgenic Arabidopsis expressing a TPK1-green fluorescent protein (GFP) fusion or protoplasts transiently transformed with chimeric constructs based on domain exchange between TPK1 and TPK4, the only TPK family member not located at the tonoplast. The results show that TPK1-GFP is a dimer and that the newly synthesized polypeptides transiently interact with a thus-far unidentified 20-kD polypeptide. A subset of the TPK1-TPK4 chimeras were unable to assemble correctly and these remained located in the endoplasmic reticulum where they interacted with the binding protein chaperone. Therefore, TPK1 must assemble correctly to pass endoplasmic reticulum quality control. Substitution of the cytosolic C terminus of TPK4 with the corresponding domain of TPK1 was sufficient to allow tonoplast delivery, indicating that this domain contains tonoplast sorting information. Pulse-chase labeling indicated that TPK1-GFP has a half-life of at least 24 h. Turnover of the fusion protein involves internalization into the vacuole where the GFP domain is released. This indicates a possible mechanism for the turnover of tonoplast proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.