ArticleMolecular biology of the cell2011
Arp2/3 promotes junction formation and maintenance in the Caenorhabditis elegans intestine by regulating membrane association of apical proteins.
Article in Molecular biology of the cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- TheMolecular biology of the cell · 2026Article
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- Branched-chain actin dynamics polarizes vesicle trajectories and partitions apicobasal epithelial membrane domains.Science advances · 2023Article
- WAVE facilitates polarized E-cadherin transport.Molecular biology of the cell · 2023Article
- A Caenorhabditis elegans nck-1 and filamentous actin-regulating protein pathway mediates a key cellular defense against bacterial pore-forming proteins.PLoS pathogens · 2022Article
- ERM-1 Phosphorylation and NRFL-1 Redundantly Control Lumen Formation in theFrontiers in cell and developmental biology · 2022Article
- Functions of Arp2/3 Complex in the Dynamics of Epithelial Tissues.Frontiers in cell and developmental biology · 2022Review
- Translation-dependent mRNA localization to Caenorhabditis elegans adherens junctions.Development (Cambridge, England) · 2021Article
- Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells.PLoS genetics · 2021Article
- E-Cadherin/HMR-1 Membrane Enrichment Is Polarized by WAVE-Dependent Branched Actin.Journal of developmental biology · 2021Article
- Epithelial morphogenesis, tubulogenesis and forces in organogenesis.Current topics in developmental biology · 2021Review
- C-terminal phosphorylation modulates ERM-1 localization and dynamics to control cortical actin organization and support lumen formation duringDevelopment (Cambridge, England) · 2020Article
- The Arp2/3 complex and the formin, Diaphanous, are both required to regulate the size of germline ring canals in the developing egg chamber.Developmental biology · 2020Article
- Actin protrusions push at apical junctions to maintain E-cadherin adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Age-Onset Phosphorylation of a Minor Actin Variant Promotes Intestinal Barrier Dysfunction.Developmental cell · 2019Article
- Paxillin-dependent regulation of apical-basal polarity in mammary gland morphogenesis.Development (Cambridge, England) · 2019Article
- WAVE regulates Cadherin junction assembly and turnover during epithelial polarization.Developmental biology · 2018Article
- Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension.Nature communications · 2016Article
- Identifying Regulators of Morphogenesis Common to Vertebrate Neural Tube Closure and Caenorhabditis elegans Gastrulation.Genetics · 2016Article
- Synaptopodin couples epithelial contractility to α-actinin-4-dependent junction maturation.The Journal of cell biology · 2015Article
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4 authors.
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Abstract
It has been proposed that Arp2/3, which promotes nucleation of branched actin, is needed for epithelial junction initiation but is less important as junctions mature. We focus here on how Arp2/3 contributes to the Caenorhabditis elegans intestinal epithelium and find important roles for Arp2/3 in the maturation and maintenance of junctions in embryos and adults. Electron microscope studies show that embryos depleted of Arp2/3 form apical actin-rich microvilli and electron-dense apical junctions. However, whereas apical/basal polarity initiates, apical maturation is defective, including decreased apical F-actin enrichment, aberrant lumen morphology, and reduced accumulation of some apical junctional proteins, including DLG-1. Depletion of Arp2/3 in adult animals leads to similar intestinal defects. The DLG-1/AJM-1 apical junction proteins, and the ezrin-radixin-moesin homologue ERM-1, a protein that connects F-actin to membranes, are required along with Arp2/3 for apical F-actin enrichment in embryos, whereas cadherin junction proteins are not. Arp2/3 affects the subcellular distribution of DLG-1 and ERM-1. Loss of Arp2/3 shifts both ERM-1 and DLG-1 from pellet fractions to supernatant fractions, suggesting a role for Arp2/3 in the distribution of membrane-associated proteins. Thus, Arp2/3 is required as junctions mature to maintain apical proteins associated with the correct membranes.
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