ArticleJournal of clinical microbiology2011
Identification of HIV superinfection in seroconcordant couples in Rakai, Uganda, by use of next-generation deep sequencing.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 64 citations in OpenAlex.
- Brief Report: Rebound HIV Viremia With Meningoencephalitis After Antiretroviral Therapy Interruption After Allogeneic Bone Marrow Transplant.Journal of acquired immune deficiency syndromes (1999) · 2022Trial
- Short Communication: Persistence of HIV After Allogeneic Bone Marrow Transplant in a Dually Infected Individual.AIDS research and human retroviruses · 2022Trial
- Limited HIV-1 superinfection in seroconverters from the CAPRISA 004 Microbicide Trial.Journal of clinical microbiology · 2014Trial
- HIV-1 superinfection occurs less frequently than initial infection in a cohort of high-risk Kenyan women.PLoS pathogens · 2013Trial
- Analysis of genetic linkage of HIV from couples enrolled in the HIV Prevention Trials Network 052 trial.The Journal of infectious diseases · 2011Trial
- HIV superinfection reveals sequential reservoir reactivation and immune-driven rebound dynamics.Journal of virology · 2026Article
- Quantifying prevalence and risk factors of HIV multiple infection in Uganda from population-based deep-sequence data.PLoS pathogens · 2025Article
- Article
- Persistence of HIV transmission clusters among people who inject drugs.AIDS (London, England) · 2020Article
- Outcomes of donor-derived superinfection screening in HIV-positive to HIV-positive kidney and liver transplantation: a multicentre, prospective, observational study.The lancet. HIV · 2020Observational
- Triple HIV-1 Infection Is Associated With Faster CD4Frontiers in microbiology · 2020Article
- Limited anti-HIV neutralizing antibody breadth and potency before and after HIV superinfection in Danish men who have sex with men.Infectious diseases (London, England) · 2019Article
- Evolution of HIV-1 within untreated individuals and at the population scale in Uganda.PLoS pathogens · 2018Article
- Characterization of the Neutralizing Antibody Response in a Case of Genetically Linked HIV Superinfection.The Journal of infectious diseases · 2018Article
- HIV-Positive Kidney Donor Selection for HIV-Positive Transplant Recipients.Journal of the American Society of Nephrology : JASN · 2018Review
- The Transmission and Evolution of HIV-1 Quasispecies within One Couple: a Follow-up Study based on Next-Generation Sequencing.Scientific reports · 2018Article
- Variable Effect of HIV Superinfection on Clinical Status: Insights From Mathematical Modeling.Frontiers in microbiology · 2018Article
- Dual-strain genital herpes simplex virus type 2 (HSV-2) infection in the US, Peru, and 8 countries in sub-Saharan Africa: A nested cross-sectional viral genotyping study.PLoS medicine · 2017Article
- Immunological and virological characteristics of human immunodeficiency virus type 1 superinfection: implications in vaccine design.Frontiers of medicine · 2017Review
- Reduced Frequency of Cells Latently Infected With Replication-Competent Human Immunodeficiency Virus-1 in Virally Suppressed Individuals Living in Rakai, Uganda.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2017Article
Corrections and comments
- Erratum issued
Authors and funding
16 authors at 5 institutions in 2 countries.
Funding
Abstract
HIV superinfection, which occurs when a previously infected individual acquires a new distinct HIV strain, has been described in a number of populations. Previous methods to detect superinfection have involved a combination of labor-intensive assays with various rates of success. We designed and tested a next-generation sequencing (NGS) protocol to identify HIV superinfection by targeting two regions of the HIV viral genome, p24 and gp41. The method was validated by mixing control samples infected with HIV subtype A or D at different ratios to determine the inter- and intrasubtype sensitivity by NGS. This amplicon-based NGS protocol was able to consistently identify distinct intersubtype strains at ratios of 1% and intrasubtype variants at ratios of 5%. By using stored samples from the Rakai Community Cohort Study (RCCS) in Uganda, 11 individuals who were HIV seroconcordant but virally unlinked from their spouses were then tested by this method to detect superinfection between 2002 and 2005. Two female cases of HIV intersubtype superinfection (18.2%) were identified. These results are consistent with other African studies and support the hypothesis that HIV superinfection occurs at a relatively high rate. Our results indicate that NGS can be used for detection of HIV superinfection within large cohorts, which could assist in determining the incidence and the epidemiologic, virologic, and immunological correlates of this phenomenon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.