ArticleJournal of clinical microbiology2011
Multilaboratory evaluation of real-time PCR tests for hepatitis B virus DNA quantification.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Article
- Performance Evaluation of the Roche Cobas 5800 HBV and HCV Tests: Comparison of the 200 and 500 μL Protocols.Annals of laboratory medicine · 2024Article
- Detection technology and clinical applications of serum viral products of hepatitis B virus infection.Frontiers in cellular and infection microbiology · 2024Review
- Electrochemical Biosensor for Sensitive Detection of Hepatitis B in Human Plasma.Applied biochemistry and biotechnology · 2022Article
- Development of sensitive and specific loop-mediated isothermal amplification combined with lateral flow device for the rapid detection of hepatitis B virus infection.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection.Scientific reports · 2022Article
- Comparison of the Amplisure HBV Quantitative Kit with the Qiagen Artus HBV QS-RGQ Assay for Quantifying Viral DNA in Plasma Samples of Monitoring Cases.Intervirology · 2021Article
- Advances in Directly Amplifying Nucleic Acids from Complex Samples.Biosensors · 2019Review
- Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood.Heliyon · 2018Article
- Performance verification and comparison of TianLong automatic hypersensitive hepatitis B virus DNA quantification system with Roche CAP/CTM system.World journal of gastroenterology · 2017Article
- Comparison of the QIAGEN artus HBV QS-RGQ Assay With the Roche COBAS AmpliPrep/COBAS TaqMan HBV Assay for Quantifying Viral DNA in Sera of Chronic Hepatitis B Patients.Annals of laboratory medicine · 2017Article
- Diagnosis of hepatitis B.Annals of translational medicine · 2016Review
- Enhancement of PCR Detection Limit by Single-Tube Restriction Endonuclease-PCR (RE-PCR).Molecular diagnosis & therapy · 2016Article
- Epidemiology and Clinical Presentations of Respiratory Syncytial Virus Subgroups A and B Detected with Multiplex Real-Time PCR.PloS one · 2016Article
- Update on hepatitis B and C virus diagnosis.World journal of virology · 2015Review
- Detection of hepatitis B virus infection: A systematic review.World journal of hepatology · 2015Article
- Rapid detection of hepatitis B virus in blood plasma by a specific and sensitive loop-mediated isothermal amplification assay.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014Article
- A multiplex two-color real-time PCR method for quality-controlled molecular diagnostic testing of FFPE samples.PloS one · 2014Article
- Performance evaluation of ExiStation HBV diagnostic system for hepatitis B virus DNA quantitation.Journal of virological methods · 2013Article
- Comparison of three Roche hepatitis B virus viral load assay formats.Journal of clinical microbiology · 2012Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The performance characteristics of four different assays for hepatitis B virus (HBV) quantification were assessed: the Abbott RealTime HBV IUO, the Roche Cobas AmpliPrep/Cobas TaqMan HBV test, the Roche Cobas TaqMan HBV test with HighPure system, and the Qiagen artus HBV TM ASR. Limit of detection (LOD), linear range, reproducibility, and agreement were determined using a serially diluted plasma sample from a single chronically infected subject. Each assay was tested by at least three laboratories. The LOD of the RealTime and two TaqMan assays was approximately 1.0 log(10) IU/ml; for artus HBV (which used the lowest volume of extracted DNA), it was approximately 1.5 log(10) IU/ml. The linear range spanned 1.0 to at least 7.0 log(10) IU/ml for all assays. Median values were consistently lowest for artus HBV and highest for Cobas AmpliPrep/Cobas TaqMan HBV. Assays incorporating automated nucleic acid extraction were the most reproducible; however, the overall variability was minor since the standard deviations for the means of all tested concentrations were ≤0.32 log(10) IU/ml for all assays. False-positive results were observed with all assays; the highest rates occurred with tests using manual nucleic acid extraction. The performance characteristics of these assays suggest that they are useful for management and therapeutic monitoring of chronic HBV infection.
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