ArticleAnalytical chemistry2011
High-throughput screening of drug-lipid membrane interactions via counter-propagating second harmonic generation imaging.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 61 citations in OpenAlex.
- Second harmonic imaging of membrane potentials: Comparing water and chromophores as probes.Biophysical journal · 2026Article
- Monitoring Molecular Interactions with Cell Membranes Using Time-Dependent Second Harmonic Generation Microscopy.Biochemistry · 2025Article
- Miltefosine impacts small molecule transport in Gram-positive bacteria.RSC chemical biology · 2024Article
- Facilitating flip-flop: Structural tuning of molecule-membrane interactions in living bacteria.Biophysical journal · 2023Article
- Passive transport of CaBiophysical journal · 2023Article
- Monitoring membranes: The exploration of biological bilayers with second harmonic generation.Chemical physics reviews · 2022Review
- Revealing the Wonder of Natural Photonics by Nonlinear Optics.Biomimetics (Basel, Switzerland) · 2022Article
- Substrate-supported Model Membrane as a Versatile Analytical/Biosensing Platform.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Label-free and charge-sensitive dynamic imaging of lipid membrane hydration on millisecond time scales.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Measuring selective estrogen receptor modulator (SERM)-membrane interactions with second harmonic generation.Journal of the American Chemical Society · 2014Article
- Lens-less surface second harmonic imaging.Optics express · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Here we report the use of counter-propagating second harmonic generation (SHG) to image the interactions between the local anesthetic tetracaine and a multicomponent planar supported lipid bilayer array in a label-free manner. The lipid bilayer arrays, prepared using a 3D continuous flow microspotter, allow the effects of lipid phase and cholesterol content on tetracaine binding to be examined simultaneously. SHG images show that tetracaine has a higher binding affinity to liquid-crystalline phase lipids than to solid-gel phase lipids. The presence of 28 mol % cholesterol decreased the binding affinity of tetracaine to bilayers composed of the mixed chain lipid, 1-steroyl-2-oleoyl-sn-glycero-3-phophocholine (SOPC), and the saturated lipids 1,2-dimyristoyl-sn-glycero-3-phophocholine (DMPC) and 1,2-dipamitoyl-sn-glycero-3-phophocholine (DPPC) while having no effect on diunsaturated 1,2-dioleoyl-sn-glycero-3-phophocholine (DOPC). The maximum surface excess of tetracaine increases with the degree of unsaturation of the phospholipids and decreases with cholesterol in the lipid bilayers. The paper demonstrates that SHG imaging is a sensitive technique that can directly image and quantitatively measure the association of a drug to a multicomponent lipid bilayer array, providing a high-throughput means to assess drug-membrane interactions.
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Registered trials
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