ArticlePloS one2011
Molecular strategy for survival at a critical high temperature in Eschierichia coli.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 102 citations in OpenAlex.
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- Characterization of a Novel RNA Polymerase Mutant of Escherichia coli That Confers Thermotolerance and Chaperone Independence.Molecular microbiology · 2025Article
- Prior exposure strongly influences mechanisms underpinning survival of heat shock inFrontiers in microbiology · 2025Article
- Lipopolysaccharide Core Truncation in InvasiveInternational journal of molecular sciences · 2024Article
- Co-ordinated assembly of the multilayered cell envelope of Gram-negative bacteria.Current opinion in microbiology · 2024Review
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- Integration of (S)-2,3-oxidosqualene enables E. coli to become Iron Man E. coli with improved overall tolerance.Biotechnology for biofuels and bioproducts · 2023Article
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- Purification and Characterization of Authentic 30S Ribosomal Precursors Induced by Heat Shock.International journal of molecular sciences · 2023Article
- Bacteriophage Infection of the Marine BacteriumMicroorganisms · 2023Article
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- MetA is a "thermal fuse" that inhibits growth and protects Escherichia coli at elevated temperatures.Cell reports · 2022Article
- A New Factor LapD Is Required for the Regulation of LpxC Amounts and Lipopolysaccharide Trafficking.International journal of molecular sciences · 2022Article
- Proteomic Profiling of Outer Membrane Vesicles Released byJournal of personalized medicine · 2022Article
- Distinct Metabolic Flow in Response to Temperature in Thermotolerant Kluyveromyces marxianus.Applied and environmental microbiology · 2022Article
- Microbial adaptation to different environmental conditions: molecular perspective of evolved genetic and cellular systems.Archives of microbiology · 2022Review
- Growth and genome-based insights of Fe(III) reduction of the high-temperature and NaCl-tolerantFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli was investigated. Genome-wide screening with a single-gene knockout library provided a list of genes indispensable for growth at 47°C, called thermotolerant genes. Genes for which expression was affected by exposure to CHT were identified by DNA chip analysis. Unexpectedly, the former contents did not overlap with the latter except for dnaJ and dnaK, indicating that a specific set of non-heat shock genes is required for the organism to survive under such a severe condition. More than half of the mutants of the thermotolerant genes were found to be sensitive to H(2)O(2) at 30°C, suggesting that the mechanism of thermotolerance partially overlaps with that of oxidative stress resistance. Their encoded enzymes or proteins are related to outer membrane organization, DNA double-strand break repair, tRNA modification, protein quality control, translation control or cell division. DNA chip analyses of essential genes suggest that many of the genes encoding ribosomal proteins are down-regulated at CHT. Bioinformatics analysis and comparison with the genomic information of other microbes suggest that E. coli possesses several systems for survival at CHT. This analysis allows us to speculate that a lipopolysaccharide biosynthesis system for outer membrane organization and a sulfur-relay system for tRNA modification have been acquired by horizontal gene transfer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.