ArticlePloS one2011
HuB (elavl2) mRNA is restricted to the germ cells by post-transcriptional mechanisms including stabilisation of the message by DAZL.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Cell-type-specific mRNA transcription and degradation kinetics in zebrafish embryogenesis from metabolically labeled single-cell RNA-seq.Nature communications · 2024Article
- Sex Lethal Gene Manipulates Gonadal Development of Medaka,International journal of molecular sciences · 2022Article
- The translational landscape of ground state pluripotency.Nature communications · 2020Article
- RNA immunoprecipitation identifies novel targets of DAZL in human foetal ovary.Molecular human reproduction · 2017Article
- Methods to study maternal regulation of germ cell specification in zebrafish.Methods in cell biology · 2016Article
- Hsa-miR-520d converts fibroblasts into CD105+ populations.Drugs in R&D · 2014Article
- Testicular niche required for human spermatogonial stem cell expansion.Stem cells translational medicine · 2014Article
- Conserved mechanisms for germ cell-specific localization of nanos3 transcripts in teleost species with aquaculture significance.Marine biotechnology (New York, N.Y.) · 2014Article
- Brf1 posttranscriptionally regulates pluripotency and differentiation responses downstream of Erk MAP kinase.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- An oocyte-specific ELAVL2 isoform is a translational repressor ablated from meiotically competent antral oocytes.Cell cycle (Georgetown, Tex.) · 2014Article
- Kinked β-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Specificity factors in cytoplasmic polyadenylation.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of germ cells to carry out a gene regulatory program distinct from the surrounding somatic tissue, and their capacity to specify an entire new organism has made them a focus of many studies that seek to understand how specific regulatory mechanisms, particularly post-transcriptional mechanisms, contribute to cell fate. In zebrafish, germ cells are specified through the inheritance of cytoplasmic determinants, termed the germ plasm, which contains a number of maternal mRNAs and proteins. Investigation of several of these messages has revealed that the restricted localisation of these mRNAs to the germ plasm and subsequent germ cells is due to cis-acting sequence elements present in their 3'UTRs. Here we show that a member of the Hu family of RNA-binding proteins, HuB, is maternally provided in the zebrafish embryo and exhibits germ cell specific expression during embryogenesis. Restriction of HuB mRNA to the germ cells is dependent on a number of sequence elements in its 3'UTR, which act to degrade the mRNA in the soma and stabilise it in the germ cells. In addition, we show that the germ cell specific RNA-binding protein DAZL is able to promote HuB mRNA stability and translation in germ cells, and further demonstrate that these activities require a 30 nucleotide element in the 3'UTR. Our study suggests that DAZL specifically binds the HuB 3'UTR and protects the message from degradation and/or enhances HuB translation, leading to the germ cell specific expression of HuB protein.
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