ArticlePLoS biology2011
Premitotic assembly of human CENPs -T and -W switches centromeric chromatin to a mitotic state.
Article in PLoS biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
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Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
- Sex-stratified genome-wide association study of multisite chronic pain in UK Biobank.PLoS genetics · 2021Pooled it
- Structure and function of the centromere and inner kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Cohesin-mediated stabilization of the CCAN complex at kinetochores in mitosis.Current biology : CB · 2025Article
- p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A.Molecular biology of the cell · 2023Article
- LncRNA CCTT-mediated RNA-DNA and RNA-protein interactions facilitate the recruitment of CENP-C to centromeric DNA during kinetochore assembly.Molecular cell · 2022Article
- InvestigatingFrontiers in genetics · 2022Article
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- CENP-A: A Histone H3 Variant with Key Roles in Centromere Architecture in Healthy and Diseased States.Results and problems in cell differentiation · 2022Article
- Kinetochore assembly throughout the cell cycle.Seminars in cell & developmental biology · 2021Article
- The centromere comes into focus: from CENP-A nucleosomes to kinetochore connections with the spindle.Open biology · 2020Review
- Article
- Holliday junction recognition protein interacts with and specifies the centromeric assembly of CENP-T.The Journal of biological chemistry · 2019Article
- Comparative analysis of histologically classified oligodendrogliomas reveals characteristic molecular differences between subgroups.BMC cancer · 2018Article
- Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion.Molecular medicine reports · 2018Article
- Article
- α-amino trimethylation of CENP-A by NRMT is required for full recruitment of the centromere.Nature communications · 2017Article
- Chromatin dynamics during the cell cycle at centromeres.Nature reviews. Genetics · 2017Review
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- A Molecular View of Kinetochore Assembly and Function.Biology · 2017Review
- Human centromere repositioning within euchromatin after partial chromosome deletion.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centromeres are differentiated chromatin domains, present once per chromosome, that direct segregation of the genome in mitosis and meiosis by specifying assembly of the kinetochore. They are distinct genetic loci in that their identity in most organisms is determined not by the DNA sequences they are associated with, but through specific chromatin composition and context. The core nucleosomal protein CENP-A/cenH3 plays a primary role in centromere determination in all species and directs assembly of a large complex of associated proteins in vertebrates. While CENP-A itself is stably transmitted from one generation to the next, the nature of the template for centromere replication and its relationship to kinetochore function are as yet poorly understood. Here, we investigate the assembly and inheritance of a histone fold complex of the centromere, the CENP-T/W complex, which is integrated with centromeric chromatin in association with canonical histone H3 nucleosomes. We have investigated the cell cycle regulation, timing of assembly, generational persistence, and requirement for function of CENPs -T and -W in the cell cycle in human cells. The CENP-T/W complex assembles through a dynamic exchange mechanism in late S-phase and G2, is required for mitosis in each cell cycle and does not persist across cell generations, properties reciprocal to those measured for CENP-A. We propose that the CENP-A and H3-CENP-T/W nucleosome components of the centromere are specialized for centromeric and kinetochore activities, respectively. Segregation of the assembly mechanisms for the two allows the cell to switch between chromatin configurations that reciprocally support the replication of the centromere and its conversion to a mitotic state on postreplicative chromatin.
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