ArticleNucleic acids research2011
Intrachromosomal tandem duplication and repeat expansion during attempts to inactivate the subtelomeric essential gene GSH1 in Leishmania.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Ascorbate-Dependent Peroxidase (APX) from Leishmania amazonensis Is a Reactive Oxygen Species-Induced Essential Enzyme That Regulates Virulence.Infection and immunity · 2019Article
- Identification of divergent Leishmania (Viannia) braziliensis ecotypes derived from a geographically restricted area through whole genome analysis.PLoS neglected tropical diseases · 2019Article
- Genomic insights into virulence mechanisms of Leishmania donovani: evidence from an atypical strain.BMC genomics · 2018Article
- Genomic Analysis of Colombian Leishmania panamensis strains with different level of virulence.Scientific reports · 2018Article
- Article
- Whole genome sequencing of live attenuated Leishmania donovani parasites reveals novel biomarkers of attenuation and enables product characterization.Scientific reports · 2017Article
- Chromosomal Translocations in the Parasite Leishmania by a MRE11/RAD50-Independent Microhomology-Mediated End Joining Mechanism.PLoS genetics · 2016Article
- Disclosing the essentiality of ribose-5-phosphate isomerase B in Trypanosomatids.Scientific reports · 2016Article
- A Trypanosomatid Iron Transporter that Regulates Mitochondrial Function Is Required for Leishmania amazonensis Virulence.PLoS pathogens · 2016Article
- Drug resistance analysis by next generation sequencing in Leishmania.International journal for parasitology. Drugs and drug resistance · 2015Review
- Roles of Rad51 paralogs for promoting homologous recombination in Leishmania infantum.Nucleic acids research · 2015Article
- Intrachromosomal amplification, locus deletion and point mutation in the aquaglyceroporin AQP1 gene in antimony resistant Leishmania (Viannia) guyanensis.PLoS neglected tropical diseases · 2015Article
- Genome-wide stochastic adaptive DNA amplification at direct and inverted DNA repeats in the parasite Leishmania.PLoS biology · 2014Article
- DNA repair pathways in trypanosomatids: from DNA repair to drug resistance.Microbiology and molecular biology reviews : MMBR · 2014Review
- LeishMicrosatDB: open source database of repeat sequences detected in six fully sequenced Leishmania genomes.Database : the journal of biological databases and curation · 2014Article
- Article
- Predominance of Leishmania major and rare occurrence of Leishmania tropica with haplotype variability at the center of Iran.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
- Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Gamma-glutamylcysteine synthetase encoded by GSH1 is the rate-limiting enzyme in the biosynthesis of glutathione and trypanothione in Leishmania. Attempts to generate GSH1 null mutants by gene disruption failed in Leishmania infantum. Removal of even a single allele invariably led to the generation of an extra copy of GSH1, maintaining two intact wild-type alleles. In the second and even third round of inactivation, the markers integrated at the homologous locus but always preserved two intact copies of GSH1. We probed into the mechanism of GSH1 duplication. GSH1 is subtelomeric on chromosome 18 and Southern blot analysis indicated that a 10-kb fragment flanked by 466-bp direct repeated sequences was duplicated in tandem on the same chromosomal allele each time GSH1 was targeted. Polymerase chain reaction analysis and sequencing confirmed the generation of novel junctions created at the level of the 466-bp repeats consequent to locus duplication. In loss of heterozygosity attempts, the same repeated sequences were utilized for generating extrachromosomal circular amplicons. Our results are consistent with break-induced replication as a mechanism for the generation of this regional polyploidy to compensate for the inactivation of an essential gene. This chromosomal repeat expansion through repeated sequences could be implicated in locus duplication in Leishmania.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.