ArticleVirology journal2011
Genotyping the hepatitis B virus with a fragment of the HBV DNA polymerase gene in Shenyang, China.
Article in Virology journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- High Prevalence of Hepatitis B Virus Infection Among People Living with Advanced HIV Disease in Botswana.Biomedicines · 2026Article
- Hepatitis B Virus Prevalence among HIV-Uninfected People Living in Rural and Peri-Urban Areas in Botswana.Microorganisms · 2024Article
- A Recent Prevalence of Hepatitis B Virus (HBV) Genotypes and Subtypes in Asia: A Systematic Review and Meta-Analysis.Healthcare (Basel, Switzerland) · 2023Review
- Molecular Epidemiological Characteristics and Risk Factors for Acquiring HBV Among Li Ethnic in Baisha County, Hainan Island-Subgenotype D3 Was First Discovered in China.Frontiers in microbiology · 2022Article
- Article
- Hepatitis B virus DNA polymerase gene polymorphism based prediction of genotypes in chronic HBV patients from Western India.African health sciences · 2017Article
- Slow CD4Open forum infectious diseases · 2016Article
- Association between clinical features and YMDD mutations in patients with chronic hepatitis B following lamivudine therapy.Experimental and therapeutic medicine · 2016Article
- Molecular characterisation of hepatitis B virus in HIV-1 subtype C infected patients in Botswana.BMC infectious diseases · 2015Article
- Sequence-based genotyping of hepatitis B virus in general population.Medical journal of the Islamic Republic of Iran · 2015Article
- Subgenotypes and mutations in the s and polymerase genes of hepatitis B virus carriers in the West Bank, palestine.PloS one · 2014Article
- Binding sensitivity of adefovir to the polymerase from different genotypes of HBV: molecular modeling, docking and dynamics simulation studies.Acta pharmacologica Sinica · 2013Article
- Hepatitis B surface antigen variants in voluntary blood donors in Nanjing, China.Virology journal · 2012Article
- Is there any value to hepatitis B virus genotype analysis?Current gastroenterology reports · 2012Review
- Association of glutathione S-transferase T1 and M1 genotypes with chronic liver diseases among Filipinos.International journal of molecular epidemiology and genetics · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hepatitis B virus (HBV) has been classified into eight genotypes (A-H) based on intergenotypic divergence of at least 8% in the complete nucleotide sequence or more than 4% in the S gene. To facilitate the investigation of the relationship between the efficacy of drug treatment and the mutation with specific genotype of HBV, we have established a new genotyping strategy based on a fragment of the HBV DNA polymerase gene. Pairwise sequence and phylogenetic analyses were performed using CLUSTAL V (DNASTAR) on the eight (A-H) standard full-length nucleotide sequences of HBV DNA from GenBank (NCBI) and the corresponding semi-nested PCR products from the HBV DNA polymerase gene. The differences in the semi-nested PCR fragments of the polymerase genes among genotypes A through F were greater than 4%, which is consistent with the intergenotypic divergence of at least 4% in HBV DNA S gene sequences. Genotyping using the semi-nested PCR products of the DNA polymerase genes revealed that only genotypes B, C, and D were present in the 50 cases, from Shenyang, China, with a distribution of 11 cases (22%), 25 cases (50%), and 14 cases (28%) respectively. These results demonstrate that our new genotyping method utilizing a fragment of the HBV DNA polymerase gene is valid and can be employed as a general genotyping strategy in areas with prevalent HBV genotypes A through F. In Shenyang, China, genotypes C, B, and D were identified with this new genotyping method, and genotype C was demonstrated to be the dominant genotype.
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