ArticleAnalytical chemistry2011
Ultrasensitive identification of localization variants of modified peptides using ion mobility spectrometry.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Separation of Isomeric Tau Phosphopeptides from Alzheimer's Disease Brain by Cyclic Ion Mobility Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2023Article
- Composite Multidimensional Ion Mobility-Mass Spectrometry for Improved Differentiation of Stereochemical Modifications.Analytical chemistry · 2023Article
- Identification of Mobility-ResolvedAnalytical chemistry · 2022Article
- Improved Sensitivity and Separations for Phosphopeptides using Online Liquid Chromotography Coupled with Structures for Lossless Ion Manipulations Ion Mobility-Mass Spectrometry.Analytical chemistry · 2018Article
- Linear and Differential Ion Mobility Separations of Middle-Down Proteoforms.Analytical chemistry · 2018Article
- Fast and Effective Ion Mobility-Mass Spectrometry Separation of d-Amino-Acid-Containing Peptides.Analytical chemistry · 2017Article
- Distinguishing d- and l-aspartic and isoaspartic acids in amyloid β peptides with ultrahigh resolution ion mobility spectrometry.Chemical communications (Cambridge, England) · 2017Article
- Characterization of Complete Histone Tail Proteoforms Using Differential Ion Mobility Spectrometry.Analytical chemistry · 2017Article
- A Priori Intrinsic PTM Size Parameters for Predicting the Ion Mobilities of Modified Peptides.Journal of the American Society for Mass Spectrometry · 2017Article
- Localization of Post-Translational Modifications in Peptide Mixtures via High-Resolution Differential Ion Mobility Separations Followed by Electron Transfer Dissociation.Journal of the American Society for Mass Spectrometry · 2016Article
- Ion Mobility Separations of Isomers based upon Long Path Length Structures for Lossless Ion Manipulations Combined with Mass Spectrometry.ChemistrySelect · 2016Article
- Spatial Ion Peak Compression and its Utility in Ion Mobility Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- Examining the Influence of Phosphorylation on Peptide Ion Structure by Ion Mobility Spectrometry-Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- Current peptidomics: applications, purification, identification, quantification, and functional analysis.Proteomics · 2015Review
- Automated deconvolution of overlapped ion mobility profiles.Journal of the American Society for Mass Spectrometry · 2014Article
- Experimental evaluation and optimization of structures for lossless ion manipulations for ion mobility spectrometry with time-of-flight mass spectrometry.Analytical chemistry · 2014Article
- Intrinsic Size Parameters for Palmitoylated and Carboxyamidomethylated Peptides.International journal of mass spectrometry · 2014Article
- The power of ion mobility-mass spectrometry for structural characterization and the study of conformational dynamics.Nature chemistry · 2014Review
- Site-specific characterization of (D)-amino acid containing peptide epimers by ion mobility spectrometry.Analytical chemistry · 2014Article
- High-resolution differential ion mobility spectrometry of a protein.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Localization of the modification sites on peptides is challenging, particularly when multiple modifications or mixtures of localization isomers (variants) are involved. Such variants commonly coelute in liquid chromatography and may be undistinguishable in tandem mass spectrometry (MS/MS) for lack of unique fragments. Here, we have resolved the variants of singly and doubly phosphorylated peptides employing drift tube ion mobility spectrometry (IMS) coupled to time-of-flight mass spectrometry. Even with a moderate IMS resolving power of ∼80-100, substantial separation was achieved for both 2+ and 3+ ions normally generated by electrospray ionization, including for the variants indistinguishable by MS/MS. Variants often exhibit a distribution of 3-D conformers, which can be adjusted for optimum IMS separation by prior field heating of ions in a funnel trap. The peak assignments were confirmed using MS/MS after IMS separation, but known species could be identified using just the ion mobility "tag". Avoiding the MS/MS step lowers the detection limit of localization variants to <100 amol, an order of magnitude better than that provided by electron transfer dissociation in an Orbitrap MS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.