ArticleIn vitro cellular & developmental biology. Animal2011
Rapid isolation of muscle-derived stem cells by discontinuous Percoll density gradient centrifugation.
Article in In vitro cellular & developmental biology. Animal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- Melatonin ameliorates age-related sarcopenia by inhibiting fibrogenic conversion of satellite cell.Molecular medicine (Cambridge, Mass.) · 2024Article
- Methods to Isolate Muscle Stem Cells for Cell-Based Cultured Meat Production: A Review.Animals : an open access journal from MDPI · 2024Review
- A modified preplate technique for efficient isolation and proliferation of mice muscle-derived stem cells.Cytotechnology · 2018Article
- Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation.BMC cell biology · 2018Article
- From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species.Scientific reports · 2017Article
- Molecular and functional heterogeneity of early postnatal porcine satellite cell populations is associated with bioenergetic profile.Scientific reports · 2017Article
- A Convenient and Efficient Method to Enrich and Maintain Highly Proliferative Human Fetal Liver Stem Cells.Rejuvenation research · 2015Article
- Discrimination between lung homeostatic and injury-induced epithelial progenitor subsets by cell-density properties.Stem cells and development · 2013Article
- Isolation, characterization, and differentiation of stem cells for cartilage regeneration.Annals of biomedical engineering · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated relationship between the maturity and density of muscle cells and developed a rapid isolation method to acquire stem cells from skeletal muscle. Mononuclear cells were isolated from the lower hind-limb muscles of 7-d-old male Sprague-Dawley rats and separated by Percoll density gradient centrifugation. After centrifugation, the cells were layered in the interfaces between each Percoll density layer. Flow cytometry was used to investigate the Sca-1, Pax7, CD34, CD45, M-cadherin, and myosin expression of the cells in each density layer. We found that CD45-positive cells were not present in freshly isolated muscle cells. CD34-, Pax7-positive cells were mainly observed at the interface between the 15% and 25% Percoll layers and had a density of 1.0235-1.0355 g/ml. Cells positive for M-cadherin were at the 25-35% Percoll density interface and had a density of 1.0355-1.0492 g/ml. We conclude that because there appears to be a correlation between maturity and density, muscle-derived stem cells may be isolated successfully from the 15-25% Percoll interface.
Indexed as
Identifiers
21691920What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.