ReviewPharmaceutical research2011
Quantitative glucose and ATP sensing in mammalian cells.
Review in Pharmaceutical research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- A pilot study on the biochemical effects of repeated administration of 24% oral sucrose vs. 30% oral dextrose on urinary markers of adenosine triphosphate degradation.Journal of perinatology : official journal of the California Perinatal Association · 2021Trial
- Experimental and computational investigation of the kinetic evolution of the glutaminolysis pathway and its interplay with the glycolysis pathway.FEBS open bio · 2024Article
- Mitochondrial Dynamics and Insulin Secretion.International journal of molecular sciences · 2023Review
- Deoxynivalenol affects cell metabolism in vivo and inhibits protein synthesis in IPEC-1 cells.Mycotoxin research · 2023Article
- Ketogenic Diet and Ketone Bodies against Ischemic Injury: Targets, Mechanisms, and Therapeutic Potential.International journal of molecular sciences · 2023Review
- Review
- Activation of TGR5 Ameliorates Streptozotocin-Induced Cognitive Impairment by Modulating Apoptosis, Neurogenesis, and Neuronal Firing.Oxidative medicine and cellular longevity · 2022Article
- Considerations for using isolated cell systems to understand cardiac metabolism and biology.Journal of molecular and cellular cardiology · 2021Review
- Importance of the Microbiota Inhibitory Mechanism on the Warburg Effect in Colorectal Cancer Cells.Journal of gastrointestinal cancer · 2020Review
- A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells.International journal of molecular sciences · 2020Article
- Spatial heterogeneity in the mammalian liver.Nature reviews. Gastroenterology & hepatology · 2019Review
- Mitochondrial Morphofunction in Mammalian Cells.Antioxidants & redox signaling · 2019Review
- A Microfluidic-Based Microscopy Platform for Continuous Interrogation of Trypanosoma brucei during Environmental Perturbation.Biochemistry · 2019Article
- Live-Imaging Readouts and Cell Models for Phenotypic Profiling of Mitochondrial Function.Frontiers in genetics · 2019Review
- A FRET flow cytometry method for monitoring cytosolic and glycosomal glucose in living kinetoplastid parasites.PLoS neglected tropical diseases · 2018Article
- Synthesis of newBeilstein journal of organic chemistry · 2018Article
- Rhodamine-based sensor for real-time imaging of mitochondrial ATP in living fibroblasts.Biochimica et biophysica acta. Bioenergetics · 2017Article
- Use of luciferase probes to measure ATP in living cells and animals.Nature protocols · 2017Article
- Imaging extracellular ATP with a genetically-encoded, ratiometric fluorescent sensor.PloS one · 2017Article
- Mitochondrial disorders in children: toward development of small-molecule treatment strategies.EMBO molecular medicine · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functioning and survival of mammalian cells requires an active energy metabolism. Metabolic dysfunction plays an important role in many human diseases, including diabetes, cancer, inherited mitochondrial disorders, and metabolic syndrome. The monosaccharide glucose constitutes a key source of cellular energy. Following its import across the plasma membrane, glucose is converted into pyruvate by the glycolysis pathway. Pyruvate oxidation supplies substrates for the ATP-generating mitochondrial oxidative phosphorylation (OXPHOS) system. To gain cell-biochemical knowledge about the operation and regulation of the cellular energy metabolism in the healthy and diseased state, quantitative knowledge is required about (changes in) metabolite concentrations under (non) steady-state conditions. This information can, for instance, be used to construct more realistic in silico models of cell metabolism, which facilitates understanding the consequences of metabolic dysfunction as well as on- and off-target effects of mitochondrial drugs. Here we review the current state-of-the-art live-cell quantification of two key cellular metabolites, glucose and ATP, using protein-based sensors. The latter apply the principle of FRET (fluorescence resonance energy transfer) and allow measurements in different cell compartments by fluorescence microscopy. We further summarize the properties and applications of the FRET-based sensors, their calibration, pitfalls, and future perspectives.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.