ArticleProceedings of the National Academy of Sciences of the United States of America2011
Protein crystallization facilitated by molecularly imprinted polymers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 135 citations in OpenAlex.
- Investigating the Effects of Nanogels in Promoting Protein Crystallization.International journal of molecular sciences · 2026Article
- Nucleation of protein crystals in pores and their growth.Biophysical reviews · 2025Review
- Universality of Hair as a Nucleant: Exploring the Effects of Surface Chemistry and Topography.Crystal growth & design · 2023Article
- Crystallisation and characterisation of muscle proteins: a mini-review.Journal of muscle research and cell motility · 2023Review
- Enhancing Protein Crystal Nucleation Using In Situ Templating on Bioconjugate-Functionalized Nanoparticles and Machine Learning.ACS applied materials & interfaces · 2023Article
- Heterogeneous Nucleation in Protein Crystallization.Biomimetics (Basel, Switzerland) · 2023Review
- Protein Crystals Nucleated and Grown by Means of Porous Materials Display Improved X-ray Diffraction Quality.International journal of molecular sciences · 2022Article
- On-line monitoring of the dopamine-based molecular imprinting processes for protein templates with the assistance of a fluorescent indicator.Mikrochimica acta · 2022Article
- Role of Self-Assembled Surface Functionalization on Nucleation Kinetics and Oriented Crystallization of a Small-Molecule Drug: Batch and Thin-Film Growth of Aspirin as a Case Study.ACS applied materials & interfaces · 2021Article
- Theoretical and experimental investigation of protein crystal nucleation in pores and crevices.IUCrJ · 2021Article
- Magnetic Synthetic Receptors for Selective Clean-Up in Protein Biomarker Quantification.Journal of proteome research · 2020Article
- Innovation versus practice in biological crystallization.Acta crystallographica. Section F, Structural biology communications · 2020Article
- Protein crystal structure determination with the crystallophore, a nucleating and phasing agent.Journal of applied crystallography · 2019Article
- Evaluation of Molecularly Imprinted Polymers as Synthetic Virus Neutralizing Antibody Mimics.Frontiers in bioengineering and biotechnology · 2019Article
- Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effects, phasing properties, and luminescence.Chemical science · 2017Article
- Generation of ribosome imprinted polymers for sensitive detection of translational responses.Scientific reports · 2017Article
- Protein crystal nucleation in pores.Scientific reports · 2017Article
- Automating the application of smart materials for protein crystallization.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Biomimetic receptors for bioanalyte detection by quartz crystal microbalances - from molecules to cells.Sensors (Basel, Switzerland) · 2014Article
- Optimization of crystallization conditions for biological macromolecules.Acta crystallographica. Section F, Structural biology communications · 2014Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
We present a previously undescribed initiative and its application, namely the design of molecularly imprinted polymers (MIPs) for producing protein crystals that are essential for determining high-resolution 3D structures of proteins. MIPs, also referred to as "smart materials," are made to contain cavities capable of rebinding protein; thus the fingerprint of the protein created on the polymer allows it to serve as an ideal template for crystal formation. We have shown that six different MIPs induced crystallization of nine proteins, yielding crystals in conditions that do not give crystals otherwise. The incorporation of MIPs in screening experiments gave rise to crystalline hits in 8-10% of the trials for three target proteins. These hits would have been missed using other known nucleants. MIPs also facilitated the formation of large single crystals at metastable conditions for seven proteins. Moreover, the presence of MIPs has led to faster formation of crystals in all cases where crystals would appear eventually and to major improvement in diffraction in some cases. The MIPs were effective for their cognate proteins and also for other proteins, with size compatibility being a likely criterion for efficacy. Atomic force microscopy (AFM) measurements demonstrated specific affinity between the MIP cavities and a protein-functionalized AFM tip, corroborating our hypothesis that due to the recognition of proteins by the cavities, MIPs can act as nucleation-inducing substrates (nucleants) by harnessing the proteins themselves as templates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.