ArticleProceedings of the National Academy of Sciences of the United States of America2011
CD9 tetraspanin generates fusion competent sites on the egg membrane for mammalian fertilization.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 111 citations in OpenAlex.
- ADAM20 Participates Modestly to Fertilization as Its Absence Leads to In Vitro Hypofertility in Mouse.Cell biochemistry and function · 2026Article
- A comprehensive review about sperm-oocyte interactions and key activation factors of fertilization in mammals.The Canadian journal of urology · 2026Review
- Mapping the placental galectin-3 interactome identifies CD9 and ITGB1 as functional glycoprotein counterreceptors during syncytialization.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Molecular mechanisms leading to gamete fusion.Fukushima journal of medical science · 2025Review
- Mechanochemistry: Fundamental Principles and Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Decoding the Genes Orchestrating Egg and Sperm Fusion Reactions and Their Roles in Fertility.Biomedicines · 2024Review
- Tetraspanin proteins in membrane remodeling processes.Journal of cell science · 2024Review
- Article
- Uroplakin 1a Interacts with Regucalcin and Proteasome Subunit Beta 1.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- A Frame-by-Frame Glance at Membrane Fusion Mechanisms: From Viral Infections to Fertilization.Biomolecules · 2023Review
- High curvature promotes fusion of lipid membranes: Predictions from continuum elastic theory.Biophysical journal · 2023Article
- Juno and CD9 protein network organization in oolemma of mouse oocyte.Frontiers in cell and developmental biology · 2023Article
- Eukaryotic fertilization and gamete fusion at a glance.Journal of cell science · 2022Article
- Changes in cortical endoplasmic reticulum clusters in the fertilized mouse oocyte†.Biology of reproduction · 2022Article
- SPACA6 ectodomain structure reveals a conserved superfamily of gamete fusion-associated proteins.Communications biology · 2022Article
- The metalloprotease ADM-4/ADAM17 promotes axonal repair.Science advances · 2022Article
- Tetraspanin Cd9b plays a role in fertility in zebrafish.PloS one · 2022Article
- Signaling Proteins Recruited to the Sperm Binding Site: Role of β-Catenin and Rho A.Frontiers in cell and developmental biology · 2022Article
- Oocyte ERM and EWI Proteins Are Involved in Mouse Fertilization.Frontiers in cell and developmental biology · 2022Article
- Evolutionary, proteomic, and experimental investigations suggest the extracellular matrix of cumulus cells mediates fertilization outcomes†.Biology of reproduction · 2021Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD9 tetraspanin is the only egg membrane protein known to be essential for fertilization. To investigate its role, we have measured, on a unique acrosome reacted sperm brought in contact with an egg, the adhesion probability and strength with a sensitivity of a single molecule attachment. Probing the binding events at different locations of wild-type egg we described different modes of interaction. Here, we show that more gamete adhesion events occur on Cd9 null eggs but that the strongest interaction mode disappears. We propose that sperm-egg fusion is a direct consequence of CD9 controlled sperm-egg adhesion properties. CD9 generates adhesion sites responsible for the strongest of the observed gamete interaction. These strong adhesion sites impose, during the whole interaction lifetime, a tight proximity of the gamete membranes, which is a requirement for fusion to take place. The CD9-induced adhesion sites would be the actual location where fusion occurs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.