ArticleInfection and immunity2011
Burkholderia cenocepacia ShvR-regulated genes that influence colony morphology, biofilm formation, and virulence.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
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- Colony morphotype variation inJournal of bacteriology · 2025Review
- Quorum sensing and DNA methylation play active roles in clinicalJournal of bacteriology · 2025Article
- Identification of Burkholderia cenocepacia non-coding RNAs expressed during Caenorhabditis elegans infection.Applied microbiology and biotechnology · 2023Article
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- Options and Limitations in Clinical Investigation of Bacterial Biofilms.Clinical microbiology reviews · 2018Review
- Biosynthesis of fragin is controlled by a novel quorum sensing signal.Nature communications · 2018Article
- GvmR - A Novel LysR-Type Transcriptional Regulator Involved in Virulence and Primary and Secondary Metabolism ofFrontiers in microbiology · 2018Article
- Vanillic acid from Actinidia deliciosa impedes virulence in Serratia marcescens by affecting S-layer, flagellin and fatty acid biosynthesis proteins.Scientific reports · 2017Article
- Proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of Xanthomonas citri subsp. citri during Citrus sinensis infection.BMC microbiology · 2017Article
- Use of Synthetic Hybrid Strains To Determine the Role of Replicon 3 in Virulence of the Burkholderia cepacia Complex.Applied and environmental microbiology · 2017Article
- Discovery ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS.Frontiers in microbiology · 2016Article
- Bacterial Adaptation during Chronic Respiratory Infections.Pathogens (Basel, Switzerland) · 2015Review
- Bordetella pertussis Isolates from Argentinean Whooping Cough Patients Display Enhanced Biofilm Formation Capacity Compared to Tohama I Reference Strain.Frontiers in microbiology · 2015Article
- Alteration of the phenotypic and pathogenic patterns of Burkholderia pseudomallei that persist in a soil environment.The American journal of tropical medicine and hygiene · 2014Article
- Insights into xanthomonas axonopodis pv. citri biofilm through proteomics.BMC microbiology · 2013Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Burkholderia cenocepacia is an opportunistic pathogen that primarily infects cystic fibrosis (CF) patients. Previously, we reported that ShvR, a LysR regulator, influences colony morphology, virulence, and biofilm formation and regulates the expression of an adjacent 24-kb genomic region encoding 24 genes. In this study, we report the functional characterization of selected genes in this region. A Tn5 mutant with shiny colony morphology was identified with a polar mutation in BCAS0208, predicted to encode an acyl-coenzyme A dehydrogenase. Mutagenesis of BCAS0208 and complementation analyses revealed that BCAS0208 is required for rough colony morphology, biofilm formation, and virulence on alfalfa seedlings. It was not possible to complement with BCAS0208 containing a mutation in the catalytic site. BCAS0201, encoding a putative flavin adenine dinucleotide (FAD)-dependent oxidoreductase, and BCAS0207, encoding a putative citrate synthase, do not influence colony morphology but are required for optimum levels of biofilm formation and virulence. Both BCAS0208 and BCAS0201 contribute to pellicle formation, although individual mutations in each of these genes had no appreciable effect on pellicle formation. A mutant with a polar insertion in BCAS0208 was significantly less virulent in a rat model of chronic lung infection as well as in the alfalfa model. Genes in this region were shown to influence utilization of branched-chain fatty acids, tricarboxylic acid cycle substrates, l-arabinose, and branched-chain amino acids. Together, our data show that the ShvR-regulated genes BCAS0208 to BCAS0201 are required for the rough colony morphotype, biofilm and pellicle formation, and virulence in B. cenocepacia.
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