ArticleInfection and immunity2011
Proteomics and pathway analyses of the milk fat globule in sheep naturally infected by Mycoplasma agalactiae provide indications of the in vivo response of the mammary epithelium to bacterial infection.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 63 citations in OpenAlex.
- Real-time PCR-based detection of Mycoplasma agalactiae in sheep bulk tank milk to support flock-level epidemiology.Veterinary research communications · 2026Article
- Appraisal of the Use of Proteomics Methodological Approaches and Technologies on Sheep and Goat Research and Clinical Work.Animals : an open access journal from MDPI · 2025Article
- The application of omics technologies for understanding tropical plants-based bioactive compounds in ruminants: a review.Journal of animal science and biotechnology · 2024Review
- Scientometrics Evaluation of Published Scientific Papers on the Use of Proteomics Technologies in Mastitis Research in Ruminants.Pathogens (Basel, Switzerland) · 2024Article
- Comprehensive proteomic analysis reveals omega-3 fatty acids to counteract endotoxin-stimulated metabolic dysregulation in porcine enterocytes.Scientific reports · 2023Article
- Unique Peptides of Cathelicidin-1 in the Early Detection of Mastitis-In Silico Analysis.International journal of molecular sciences · 2023Article
- Eating the Enemy: Mycoplasma Strategies to Evade Neutrophil Extracellular Traps (NETs) Promoting Bacterial Nucleotides Uptake and Inflammatory Damage.International journal of molecular sciences · 2022Review
- Potential Novel Biomarkers for Mastitis Diagnosis in Sheep.Animals : an open access journal from MDPI · 2021Review
- Applied Proteomics in 'One Health'.Proteomes · 2021Review
- Expression Pattern of Cathelicidins in Dairy Cows During Endometritis and Role of Bovine Endometrial Epithelial Cells in Production of Cathelicidins.Frontiers in veterinary science · 2021Article
- Novel Biomarkers of Mastitis in Goat Milk Revealed by MALDI-TOF-MS-Based Peptide Profiling.Biology · 2020Article
- Integrated Transcriptomic and Proteomic Analyses of the Interaction Between Chicken Synovial Fibroblasts andFrontiers in microbiology · 2020Article
- Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci.Scientific reports · 2019Article
- Use of Proteomics in the Study of Mastitis in Ewes.Pathogens (Basel, Switzerland) · 2019Review
- Compositional Dynamics of the Milk Fat Globule and Its Role in Infant Development.Frontiers in pediatrics · 2018Review
- Article
- Challenges and opportunities of bovine milk analysis by mass spectrometry.Clinical proteomics · 2016Review
- Simultaneous Identification of Potential Pathogenicity Factors of Mycoplasma agalactiae in the Natural Ovine Host by Negative Selection.Infection and immunity · 2015Article
- Neutrophil extracellular traps in sheep mastitis.Veterinary research · 2015Article
- Proteomics-driven analysis of ovine whey colostrum.PloS one · 2015Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Milk fat globules (MFGs) are vesicles released in milk as fat droplets surrounded by the endoplasmic reticulum and apical cell membranes. During formation and apocrine secretion by lactocytes, various amounts of cytoplasmic crescents remain trapped within the released vesicle, making MFGs a natural sampling mechanism of the lactating cell contents. With the aim of investigating the events occurring in the mammary epithelium during bacterial infection, the MFG proteome was characterized by two-dimensional difference gel electrophoresis (2-D DIGE), SDS-PAGE followed by shotgun liquid chromatography-tandem mass spectrometry (GeLC-MS/MS), label-free quantification by the normalized spectral abundance factor (NSAF) approach, Western blotting, and pathway analysis, using sheep naturally infected by Mycoplasma agalactiae. A number of protein classes were found to increase in MFGs upon infection, including proteins involved in inflammation and host defense, cortical cytoskeleton proteins, heat shock proteins, and proteins related to oxidative stress. Conversely, a strikingly lower abundance was observed for proteins devoted to MFG metabolism and secretion. To our knowledge, this is the first report describing proteomic changes occurring in MFGs during sheep infectious mastitis. The results presented here offer new insights into the in vivo response of mammary epithelial cells to bacterial infection and open the way to the discovery of protein biomarkers for monitoring clinical and subclinical mastitis.
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