ArticleTraffic (Copenhagen, Denmark)2011
Imaging single endocytic events reveals diversity in clathrin, dynamin and vesicle dynamics.
Article in Traffic (Copenhagen, Denmark), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Imaging nanoscale axial dynamics at the basal plasma membrane.The international journal of biochemistry & cell biology · 2023Review
- Cellular and structural insight into dynamin function during endocytic vesicle formation: a tale of 50 years of investigation.Bioscience reports · 2022Review
- Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.Nature communications · 2022Article
- Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles.Nature protocols · 2021Review
- Article
- Regulation of Clathrin-Mediated Endocytosis.Annual review of biochemistry · 2018Review
- Single Event Resolution of Plant Plasma Membrane Protein Endocytosis by TIRF Microscopy.Frontiers in plant science · 2017Article
- Coat/Tether Interactions-Exception or Rule?Frontiers in cell and developmental biology · 2016Review
- Real-time fluorescence imaging with 20 nm axial resolution.Nature communications · 2015Article
- Visualizing clathrin-mediated endocytosis of G protein-coupled receptors at single-event resolution via TIRF microscopy.Journal of visualized experiments : JoVE · 2014Article
- Imaging and modeling the dynamics of clathrin-mediated endocytosis.Cold Spring Harbor perspectives in biology · 2014Review
- Actin and dynamin2 dynamics and interplay during clathrin-mediated endocytosis.The Journal of cell biology · 2014Article
- Polarization-controlled TIRFM with focal drift and spatial field intensity correction.Biophysical journal · 2014Article
- Advances in analysis of low signal-to-noise images link dynamin and AP2 to the functions of an endocytic checkpoint.Developmental cell · 2013Article
- Divergent modes for cargo-mediated control of clathrin-coated pit dynamics.Molecular biology of the cell · 2013Article
- Geometric catalysis of membrane fission driven by flexible dynamin rings.Science (New York, N.Y.) · 2013Article
- Dynamics of clathrin-mediated endocytosis and its requirement for organelle biogenesis in Dictyostelium.Journal of cell science · 2012Article
- Clathrin and AP-1 regulate apical polarity and lumen formation during C. elegans tubulogenesis.Development (Cambridge, England) · 2012Article
- Review
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Authors and funding
3 authors.
Funding
Abstract
The dynamics of clathrin-mediated endocytosis can be assayed using fluorescently tagged proteins and total internal reflection fluorescence microscopy. Many of these proteins, including clathrin and dynamin, are soluble and changes in fluorescence intensity can be attributed either to membrane/vesicle movement or to changes in the numbers of individual molecules. It is important for assays to discriminate between physical membrane events and the dynamics of molecules. Two physical events in endocytosis were investigated: vesicle scission from the plasma membrane and vesicle internalization. Single vesicle analysis allowed the characterization of dynamin and clathrin dynamics relative to scission and internalization. We show that vesicles remain proximal to the plasma membrane for variable amounts of time following scission, and that uncoating of clathrin can occur before or after vesicle internalization. The dynamics of dynamin also vary with respect to scission. Results from assays based on physical events suggest that disappearance of fluorescence from the evanescent field should be re-evaluated as an assay for endocytosis. These results illustrate the heterogeneity of behaviors of endocytic vesicles and the importance of establishing suitable evaluation criteria for biophysical processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.