ArticlePloS one2011
Rapid turnover of 2-LTR HIV-1 DNA during early stage of highly active antiretroviral therapy.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Modelling HIV-1 2-LTR dynamics following raltegravir intensification.Journal of the Royal Society, Interface · 2013Trial
- Undetectable unintegrated HIV-DNA in persons with HIV: frequency and determinants.BMC infectious diseases · 2026Article
- Visualizing and Analyzing Global Trends and Frontier Research in HIV Reservoirs: A Bibliometric Study from 1994 to 2023.Current HIV research · 2025Article
- Validation of digital droplet PCR assays for cell-associated HIV-1 DNA, HIV-1 2-LTR circle, and HIV-1 unspliced RNA for clinical studies in HIV-1 cure research.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2024Article
- Review
- Antiretroviral (ARV) Drug Resistance and HIV-1 Subtypes among Injecting Drug Users in the Coastal Region of Kenya.Advances in virology · 2022Article
- A comparative analysis of unintegrated HIV-1 DNA measurement as a potential biomarker of the cellular reservoir in the blood of patients controlling and non-controlling viral replication.Journal of translational medicine · 2020Article
- Impact of Antiretroviral Therapy Duration on HIV-1 Infection of T Cells within Anatomic Sites.Journal of virology · 2020Article
- Increasing contribution of integrated forms to total HIV DNA in blood during HIV disease progression from primary infection.EBioMedicine · 2019Article
- Dynamics of Simian Immunodeficiency Virus Two-Long-Terminal-Repeat Circles in the Presence and Absence of CD8Journal of virology · 2018Article
- Protein expression from unintegrated HIV-1 DNA introduces bias in primary in vitro post-integration latency models.Scientific reports · 2016Article
- Order preservation of expected information content using Unscented Transform approximation of multivariate prior distributions in HIV 2-LTR experiment design.Proceedings of the ... IEEE Conference on Decision & Control. IEEE Conference on Decision & Control · 2016Article
- Persistence of integrated HIV DNA in CXCR3 + CCR6 + memory CD4+ T cells in HIV-infected individuals on antiretroviral therapy.AIDS (London, England) · 2016Article
- How to Define the Latent Reservoir: Tools of the Trade.Current HIV/AIDS reports · 2016Review
- Measuring the latent reservoir in vivo.The Journal of clinical investigation · 2016Review
- Towards an HIV-1 cure: measuring the latent reservoir.Trends in microbiology · 2015Review
- Accurate quantification of episomal HIV-1 two-long terminal repeat circles by use of optimized DNA isolation and droplet digital PCR.Journal of clinical microbiology · 2015Article
- Changes in HIV reservoirs during long-term antiretroviral therapy.Current opinion in HIV and AIDS · 2015Review
- Spatial modeling of HIV cryptic viremia and 2-LTR formation during raltegravir intensification.Journal of theoretical biology · 2014Article
- An HIV-1 replication pathway utilizing reverse transcription products that fail to integrate.Journal of virology · 2013Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite prolonged treatment with highly active antiretroviral therapy (HAART), the infectious HIV-1 continues to replicate and resides latently in the resting memory CD4+ T lymphocytes, which blocks the eradication of HIV-1. The viral persistence of HIV-1 is mainly caused by its proviral DNA being either linear nonintegrated, circular nonintegrated, or integrated. Previous reports have largely focused on the dynamics of HIV-1 DNA from the samples collected with relatively long time intervals during the process of disease and HAART treatment, which may have missed the intricate changes during the intervals in early treatment. METHODOLOGY/PRINCIPAL
findingsIn this study, we investigated the dynamics of HIV-1 DNA in patients during the early phase of HARRT treatment. Using optimized real time PCR, we observed significant changes in 2-LTR during the first 12-week of treatment, while total and integrated HIV-1 DNA remained stable. The doubling time and half-life of 2-LTR were not correlated with the baseline and the rate of changes in plasma viral load and various CD4+ T-cell populations. Longitudinal analyses on 2-LTR sequences and plasma lipopolysaccharide (LPS) levels did not reveal any significant changes in the same treatment period. CONCLUSIONS/SIGNIFICANCE: Our study revealed the rapid changes in 2-LTR concentration in a relatively large number of patients during the early HAART treatment. The rapid changes indicate the rapid infusion and clearance of cells bearing 2-LTR in the peripheral blood. Those changes are not expected to be caused by the blocking of viral integration, as our study did not include the integrase inhibitor raltegravir. Our study helps better understand the dynamics of HIV-DNA and its potential role as a biomarker for the diseases and for the treatment efficacy of HAART.
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