ArticleBacteriophage2011
Naturally resident and exogenously applied T4-like and T5-like bacteriophages can reduce Escherichia coli O157:H7 levels in sheep guts.
Article in Bacteriophage, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 75 citations in OpenAlex.
- Bacteriophage Therapy in Companion and Farm Animals.Antibiotics (Basel, Switzerland) · 2024Review
- Bacteriophages for the Targeted Control of Foodborne Pathogens.Foods (Basel, Switzerland) · 2023Review
- Isolation, characterization and whole genome analysis of the novel genus Lederbergvirus, phage vB_EcoP_E212 infecting enterotoxigenic Escherichia coli K88.Virus research · 2023Article
- Recent trends in the use of bacteriophages as replacement of antimicrobials against food-animal pathogens.Frontiers in veterinary science · 2023Review
- Characterisation of new anti-O157 bacteriophages of bovine origin representing three genera.Archives of microbiology · 2022Article
- Hypoxia Increases the Tempo of Phage Resistance and Mutational Bottlenecking ofFrontiers in microbiology · 2022Article
- The interactions of bacteriophage Ace and Shiga toxin-producing Escherichia coli during biocontrol.FEMS microbiology ecology · 2021Article
- Phages in Food Industry Biocontrol and Bioremediation.Antibiotics (Basel, Switzerland) · 2021Review
- An Assessment of the Viability of Lytic Phages and Their Potency against Multidrug ResistantAntibiotics (Basel, Switzerland) · 2021Article
- The Effect of Oxygen Availability on Bacteriophage Infection: A Review.PHAGE (New Rochelle, N.Y.) · 2021Review
- Inhibition of Antimicrobial-ResistantFrontiers in microbiology · 2021Article
- High-throughput mapping of the phage resistance landscape in E. coli.PLoS biology · 2020Article
- Encapsulation of E. coli phage ZCEC5 in chitosan-alginate beads as a delivery system in phage therapy.AMB Express · 2019Article
- Bacterial resistance to antibiotic alternatives: a wolf in sheep's clothing?Animal frontiers : the review magazine of animal agriculture · 2018Article
- Identification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products.Frontiers in microbiology · 2018Article
- Complete genome of a novel virulent phage ST0 lysingStandards in genomic sciences · 2017Article
- Efficacy of Phage Therapy in Controlling Rabbit Colibacillosis and Changes in Cecal Microbiota.Frontiers in microbiology · 2017Article
- Article
- Prevention of bacterial foodborne disease using nanobiotechnology.Nanotechnology, science and applications · 2014Review
- Tradeoffs in bacteriophage life histories.Bacteriophage · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
In preparing sheep for an in vivo Escherichia coli O157:H7 eradication trial, we found that 20/39 members of a single flock were naturally colonized by O157:H7-infecting phages. Characterization showed these were all one phage type (subsequently named CEV2) infecting 15/16 O157:H7, 7/72 ECOR and common lab strains. Further characterization by PFGE (genome∼120 kb), restriction enzyme digest (DNA appears unmodified), receptor studies (FhuA but not TonB is required for infection) and sequencing (>95% nucleotide identity) showed it is a close relative of the classically studied coliphage T5. Unlike T5, CEV2 infects O157:H7 in vitro, both aerobically and anaerobically, rapidly adsorbing and killing, but resistant mutants regrew within 24 h. When used together with T4-like CEV1 (MOI ∼2 per phage), bacterial killing was longer lasting. CEV2 did not reproduce when co-infecting the same cell as CEV1, presumably succumbing to CEV1's ability to shut off transcription of cytosine-containing DNA. In vivo sheep trials to remove resident O157:H7 showed that a cocktail of CEV2 and CEV1 (∼10(11) total PFU) applied once orally was more effective (>99.9% reduction) than CEV1 alone (∼99%) compared to the untreated phage-free control. Those sheep naturally carrying CEV2, receiving no additional phage treatment, had the lowest O157:H7 levels (∼99.99% reduction). These data suggest that phage cocktails are more effective than individual phage in removing O157:H7 that have taken residence if the phage work in concert with one another and that naturally resident O157:H7-infecting phages may prevent O157:H7 gut colonization and be one explanation for the transient O157:H7 colonization in ruminants.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.