ArticleFrontiers in microbiology2011
Molecular Characterization of Exploitation of the Polyubiquitination and Farnesylation Machineries of Dictyostelium Discoideum by the AnkB F-Box Effector of Legionella Pneumophila.
Article in Frontiers in microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- The Ecology and Evolution of Amoeba-Bacterium Interactions.Applied and environmental microbiology · 2021Review
- Effect of Bacterial Infection on the Edibility of Aquatic Products: The Case of Crayfish (Frontiers in microbiology · 2021Article
- Nutrition and Bipartite Metabolism of Intracellular Pathogens.Trends in microbiology · 2019Review
- Mammalian Solute Carrier (SLC)-like transporters of Legionella pneumophila.Scientific reports · 2018Article
- Review
- Divergent evolution of Di-lysine ER retention vs. farnesylation motif-mediated anchoring of the AnkB virulence effector to the Legionella-containing vacuolar membrane.Scientific reports · 2017Article
- Yersinia pestis Resists Predation by Acanthamoeba castellanii and Exhibits Prolonged Intracellular Survival.Applied and environmental microbiology · 2017Article
- Orientia tsutsugamushi Strain Ikeda Ankyrin Repeat-Containing Proteins Recruit SCF1 Ubiquitin Ligase Machinery via Poxvirus-Like F-Box Motifs.Journal of bacteriology · 2015Article
- Amoeba host-Legionella synchronization of amino acid auxotrophy and its role in bacterial adaptation and pathogenic evolution.Environmental microbiology · 2014Review
- Nutrient salvaging and metabolism by the intracellular pathogen Legionella pneumophila.Frontiers in cellular and infection microbiology · 2014Review
- Rapid nutritional remodeling of the host cell upon attachment of Legionella pneumophila.Infection and immunity · 2014Article
- Nutrient generation and retrieval from the host cell cytosol by intra-vacuolar Legionella pneumophila.Frontiers in cellular and infection microbiology · 2014Review
- Review
- Iron metabolism and resistance to infection by invasive bacteria in the social amoeba Dictyostelium discoideum.Frontiers in cellular and infection microbiology · 2013Review
- Exploitation of evolutionarily conserved amoeba and mammalian processes by Legionella.Trends in microbiology · 2012Review
- Lateral gene exchanges shape the genomes of amoeba-resisting microorganisms.Frontiers in cellular and infection microbiology · 2012Review
- Bacterial toxin effector-membrane targeting: outside in, then back again.Frontiers in cellular and infection microbiology · 2012Review
- ThANKs for the repeat: Intracellular pathogens exploit a common eukaryotic domain.Cellular logistics · 2011Article
- Legionella: from protozoa to humans.Frontiers in microbiology · 2011Article
- Is host lipidation of pathogen effector proteins a general virulence mechanism?Frontiers in microbiology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The Dot/Icm-translocated Ankyrin B (AnkB) F-box effector of Legionella pneumophila is essential for intra-vacuolar proliferation and functions as a platform for the docking of polyubiquitinated proteins to the Legionella-containing vacuole (LCV) within macrophages and ameba. Here we show that ectopically expressed AnkB in Dictyostelium discoideum is targeted to the plasma membrane where it recruits polyubiquitinated proteins and it trans-rescues the intracellular growth defect of the ankB null mutant, which has never been demonstrated for any effector in ameba. Using co-immunoprecipitation and bimolecular fluorescence complementation we show specific interaction of Skp1 of D. discoideum with the F-box domain of AnkB, which has never been demonstrated in ameba. We show that anchoring of AnkB to the cytosolic face of the LCV membrane in D. discoideum is mediated by the host farnesylation of the C-terminal eukaryotic CaaX motif of AnkB and is independent of the F-box and the two ANK domains, which has never been demonstrated in ameba. Importantly, the three host farnesylation enzymes farnesyl transferase, RCE-1, and isoprenyl cysteine carboxyl methyl transferase of D. discoideum are recruited to the LCV in a Dot/Icm-dependent manner, which has never been demonstrated in ameba. We conclude that the polyubiquitination and farnesylation enzymatic machineries of D. discoideum are recruited to the LCV in a Dot/Icm-dependent manner and the AnkB effector exploits the two evolutionarily conserved eukaryotic machineries to proliferate within ameba, similar to mammalian cells. We propose that L. pneumophila has acquired ankB through inter-kingdom horizontal gene transfer from primitive eukaryotes, which facilitated proliferation of L. pneumophila within human cells and the emergence of Legionnaires' disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.