ArticleInternational journal of molecular epidemiology and genetics2011
Comparison of PCR with other diagnostic techniques for the detection of H. pylori infection in patients presenting with gastroduodenal symptons in Nigeria.
Article in International journal of molecular epidemiology and genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- Prevalence of Helicobacter pylori infection among gastritis and dyspeptic patients in Jos, Plateau State, Nigeria.GMS hygiene and infection control · 2026Article
- Comparative Analysis of Diagnostic Techniques for Helicobacter pylori Infection: Insights for Effective Therapy.Journal of cellular and molecular medicine · 2025Article
- Direct detection of Helicobacter pylori from biopsies of patients in Lagos, Nigeria using real-time PCR-a pilot study.BMC research notes · 2021Article
- Stool Antigen Testing, a Reliable Noninvasive Method of Assessment of Helicobacter pylori Infection Among Patients with Gastro-duodenal Disorders in Cameroon.Digestive diseases and sciences · 2021Article
- A Comparative Study of the Adherent-InvasiveMicroorganisms · 2020Article
- Prevalence ofAfrican health sciences · 2017Article
- The immunohistochemistry and toluidine blue roles for Helicobacter pylori detection in patients with gastritis.Iranian biomedical journal · 2013Article
- A Cross-Sectional Study on Molecular Detection ofJournal of global infectious diseasesArticle
- Primary antibiotic resistance and its relationship with cagA and vacA genes in Helicobacter pylori isolates from Algerian patients.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study was aimed at comparing PCR methods of direct detection from biopsy using the boiling method and one other method with two known gold standards (histology and CLO test) for the diagnosis of H. pylori in Nigeria. A total of 168 biopsies (three from antrum and one from corpus each) were taken from 42 patients presenting with various gastroduodenal symptons after informed consent was obtained from them.The biopsies were analysed using the CLO test kit and histology, while the boiling method as described by Holmes and Quigley (1981) was used to obtain DNA and then PCR using the 16S rRNA gene, glmM gene and cagA gene. With CLO test 15/42 (35.71%) were positive, histology 13/42 (30.95%) were positive, 16S rRNA 22/42 (52.38%) were positive, glmM 19/42 (45.24%) were positive, cagA 19/42 (45.24%) were positive. The sensitivity and specificity of the PCR tests with CLO as the gold standard showed that the tests were 100% sensitive and varied between 74.1% to 84.1% in specificity. The PPV and NPV showed that the NPV was almost 100%, while the PPV was between 68.2% and 75%. Using the histology as the gold standard, the sensitivity was almost 100% while the specificity, the PPV were reduced in comparison to the CLO test. The PCR test using the glmM gene appears to be the most reliable test for diagnosis of H. pylori in Nigeria most especially where culture is difficult due to the power outages.
Indexed as
Identifiers
21686132PMC3110392W110716663What OpenQuestion holds
Registered trials
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