ArticleApplied and environmental microbiology2011
Identification of biofilm matrix-associated proteins from an acid mine drainage microbial community.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed.
- Bacterial Proteomics and Antibiotic Resistance Identification: Is Single-Cell Analysis a Worthwhile Pursuit?Pathogens (Basel, Switzerland) · 2025Review
- Targeting Bacterial Biofilms on Medical Implants: Current and Emerging Approaches.Antibiotics (Basel, Switzerland) · 2025Review
- OprF Impacts Pseudomonas aeruginosa Biofilm Matrix eDNA Levels in a Nutrient-Dependent Manner.Journal of bacteriology · 2023Article
- OprF impactsbioRxiv : the preprint server for biology · 2023Article
- Impact of c-di-GMP on the Extracellular Proteome ofBiology · 2022Article
- The evolution of alkaliphilic biofilm communities in response to extreme alkaline pH values.MicrobiologyOpen · 2022Article
- Metagenomic Mining for Esterases in the Microbial Community of Los Rueldos Acid Mine Drainage Formation.Frontiers in microbiology · 2022Article
- Microbial biofilm: formation, architecture, antibiotic resistance, and control strategies.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- Microbiome of highly polluted coal mine drainage from Onyeama, Nigeria, and its potential for sequestrating toxic heavy metals.Scientific reports · 2021Article
- Formation and characterization of biofilms formed by salt-tolerant yeast strains in seawater-based growth medium.Applied microbiology and biotechnology · 2021Article
- Proteomic Studies of the Biofilm Matrix including Outer Membrane Vesicles ofMicroorganisms · 2020Article
- Acid Mine Drainage as Habitats for Distinct Microbiomes: Current Knowledge in the Era of Molecular and Omic Technologies.Current microbiology · 2020Review
- Contributions of the Biofilm Matrix toJournal of fungi (Basel, Switzerland) · 2020Review
- Expression of metallothionein encoding gene bmtA in biofilm-forming marine bacterium Pseudomonas aeruginosa N6P6 and understanding its involvement in Pb(II) resistance and bioremediation.Environmental science and pollution research international · 2019Article
- Responses of Acinetobacter baumannii Bound and Loose Extracellular Polymeric Substances to Hyperosmotic Agents Combined with or without Tobramycin: An Atomic Force Microscopy Study.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System.Journal of visualized experiments : JoVE · 2019Article
- Human antibody repertoire frequently includes antibodies to a bacterial biofilm associated protein.PloS one · 2019Article
- Understanding the Mechanisms Behind the Response to Environmental Perturbation in Microbial Mats: A Metagenomic-Network Based Approach.Frontiers in microbiology · 2018Article
- A Human Biofilm-Disrupting Monoclonal Antibody Potentiates Antibiotic Efficacy in Rodent Models of both Staphylococcus aureus and Acinetobacter baumannii Infections.Antimicrobial agents and chemotherapy · 2017Article
- Floc Formation Reduces the pH Stress Experienced by Microorganisms Living in Alkaline Environments.Applied and environmental microbiology · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In microbial communities, extracellular polymeric substances (EPS), also called the extracellular matrix, provide the spatial organization and structural stability during biofilm development. One of the major components of EPS is protein, but it is not clear what specific functions these proteins contribute to the extracellular matrix or to microbial physiology. To investigate this in biofilms from an extremely acidic environment, we used shotgun proteomics analyses to identify proteins associated with EPS in biofilms at two developmental stages, designated DS1 and DS2. The proteome composition of the EPS was significantly different from that of the cell fraction, with more than 80% of the cellular proteins underrepresented or undetectable in EPS. In contrast, predicted periplasmic, outer membrane, and extracellular proteins were overrepresented by 3- to 7-fold in EPS. Also, EPS proteins were more basic by ∼2 pH units on average and about half the length. When categorized by predicted function, proteins involved in motility, defense, cell envelope, and unknown functions were enriched in EPS. Chaperones, such as histone-like DNA binding protein and cold shock protein, were overrepresented in EPS. Enzymes, such as protein peptidases, disulfide-isomerases, and those associated with cell wall and polysaccharide metabolism, were also detected. Two of these enzymes, identified as β-N-acetylhexosaminidase and cellulase, were confirmed in the EPS fraction by enzymatic activity assays. Compared to the differences between EPS and cellular fractions, the relative differences in the EPS proteomes between DS1 and DS2 were smaller and consistent with expected physiological changes during biofilm development.
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