ArticleMolecular & cellular proteomics : MCP2011
Integrated transcriptional and proteomic analysis with in vitro biochemical assay reveal the important role of CYP3A46 in T-2 toxin hydroxylation in porcine primary hepatocytes.
Article in Molecular & cellular proteomics : MCP, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- Multiple Mycotoxin Contamination in Livestock Feed: Implications for Animal Health, Productivity, and Food Safety.Toxins · 2025Review
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- Identification of Biotransformation Products of T-2 Toxin in HepG2 Cells Using LC-Q-TOF MS.Foods (Basel, Switzerland) · 2024Article
- Article
- Mycotoxins: Biotransformation and Bioavailability Assessment Using Caco-2 Cell Monolayer.Toxins · 2020Review
- Enzymes for Detoxification of Various Mycotoxins: Origins and Mechanisms of Catalytic Action.Molecules (Basel, Switzerland) · 2019Review
- Autophagy and Apoptosis Interact to Modulate T-2 Toxin-Induced Toxicity in Liver Cells.Toxins · 2019Article
- Impact of Subacute Exposure to T-2 Toxin and Zearalenone on the Pharmacokinetics of Midazolam as CYP3A Probe Drug in a Porcine Animal Model: A Pilot Study.Frontiers in pharmacology · 2019Article
- Conjugative Transfer of the pVA1-Type Plasmid Carrying theFrontiers in cellular and infection microbiology · 2019Article
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- Article
- Mycotoxins: cytotoxicity and biotransformation in animal cells.Toxicology research · 2016Review
- The roles of carboxylesterase and CYP isozymes on the in vitro metabolism of T-2 toxin.Military Medical Research · 2015Article
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- Chicken cytochrome P450 1A5 is the key enzyme for metabolizing T-2 toxin to 3'OH-T-2.International journal of molecular sciences · 2013Article
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Authors and funding
13 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Both T-2 toxin and its metabolites are highly potent mycotoxins that can cause severe human and animal diseases upon exposure. Understanding the toxic mechanism and biotransformation process of T-2 toxin at a cellular level is essential for the development of counter-measures. We investigated the effect of T-2 toxin in porcine primary hepatocytes using porcine genome array and two-dimensional difference gel electrophoresis with matrix-assisted laser desorption/ionization tandem time of flight mass spectrometry. Integrated transcriptional and proteomic analysis demonstrated that T-2 toxin adversely affected porcine hepatocytes by initiating lipid metabolism disorder, oxidative stress response, and apoptosis. In addition, xenobiotic metabolism genes, including cytochrome P450 3As (CYP3A46 and CYP3A39), carboxylesterase 1Cs (CES1C4 and CES1C5), and epoxide hydrolase (EPHX1), increased in T-2 toxin treatment cells. Using HepG2 cells to over-express the recombinant xenobiotic metabolism genes above and rapid resolution liquid chromatography/tandem mass spectrometry to detect metabolites of T-2 toxin, we determined that porcine CYP3A46 mainly catalyzed T-2 to form 3'-hydroxy-T-2, which was further confirmed by purified CYP3A46 protein. However, recombinant porcine CES1C5 and EPHX1 did not enhance hydrolysis and de-epoxidation of T-2 implying that other esterases and epoxide hydrolases may play dominant roles in those reactions.
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