ArticleDiagnostic microbiology and infectious disease2011
Fasciola hepatica saposin-like protein-2-based ELISA for the serodiagnosis of chronic human fascioliasis.
Article in Diagnostic microbiology and infectious disease, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 47 citations in OpenAlex.
- Production and Characterization of Recombinant Single-Chain Variable Fragment (scFv) Antibody AgainstInternational journal of molecular sciences · 2026Article
- Development of an Indirect ELISA and a Microsphere Detection Test Strip for the Detection of Spirometra mansoni.Transboundary and emerging diseases · 2026Article
- Unusual diagnosis in a Lebanese child with persistent hyper eosinophilia: a case report.Annals of medicine and surgery (2012) · 2025Article
- Article
- Diagnosis of Human Trematode Infections.Advances in experimental medicine and biology · 2024Review
- Serological diagnosis of fasciolosis (Frontiers in veterinary science · 2023Review
- Recent Developments in Recombinant Proteins for Diagnosis of Human Fascioliasis.Acta parasitologica · 2021Review
- Modulation of the Functions of Goat Peripheral Blood Mononuclear Cells byPathogens (Basel, Switzerland) · 2020Article
- Article
- Cell-free expression, purification and immunoreactivity assessment of recombinant Fasciola hepatica saposin-like protein-2.Molecular biology reports · 2018Article
- Assessment of Fasciola hepatica glutathione S-transferase as an antigen for serodiagnosis of human chronic fascioliasis.Acta tropica · 2018Article
- Identification of Immunoglobulin E-Binding Proteins of the Xerophilic Fungus Aspergillus penicillioides Crude Mycelial Mat Extract and Serological Reactivity Assessment in Subjects with Different Allergen Reactivity Profiles.International archives of allergy and immunology · 2018Article
- Evaluation of a set of refolded recombinant antigens for serodiagnosis of human fascioliasis.PloS one · 2018Article
- Immunodiagnosis of Human Fascioliasis: An Update of Concepts and Performances of the Serological Assays.Journal of clinical and diagnostic research : JCDR · 2017Review
- Serological Reactivity and Identification of Immunoglobulin E-Binding Polypeptides of Ganoderma applanatum Crude Spore Cytoplasmic Extract in Puerto Rican Subjects.International archives of allergy and immunology · 2017Article
- Development of Two FhSAP2 Recombinant-Based Assays for Immunodiagnosis of Human Chronic Fascioliasis.The American journal of tropical medicine and hygiene · 2016Article
- Adjuvant-enhanced antibody and cellular responses to inclusion bodies expressing FhSAP2 correlates with protection of mice to Fasciola hepatica.Experimental parasitology · 2016Article
- Comparative assessment of ELISAs using recombinant saposin-like protein 2 and recombinant cathepsin L-1 from Fasciola hepatica for the serodiagnosis of human Fasciolosis.PLoS neglected tropical diseases · 2014Article
- Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fascioliasis.Journal of clinical microbiology · 2014Article
- Differential expression and localization of saposin-like protein 2 of Fasciola hepatica.Acta tropica · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
An indirect enzyme-linked immunosorbent assay (ELISA) was developed and evaluated for its diagnostic ability to detect human IgG antibodies against Fasciola hepatica saposin-like protein-2. The assay was compared with an indirect ELISA with excretory-secretory products (FhES) from adult F. hepatica. In an analysis of the sera of 37 patients infected with F. hepatica, 40 patients with other parasitic infections, and 50 healthy controls, the sensitivity of both ELISA assays was 100%. However, the FhSAP2-based ELISA was more specific (95.6%) than the FhES-ELISA (91.9%). These results demonstrated that FhSAP2 can be used in the serodiagnosis of chronic human fascioliasis with the additional advantage of being relatively cheap and easy to produce. Studies are in progress to evaluate this FhSAP2-ELISA assay in a large-scale prevalence surveys in endemic areas.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.