ArticleHistochemistry and cell biology2011
Air-liquid interface cultures enhance the oxygen supply and trigger the structural and functional differentiation of intestinal porcine epithelial cells (IPEC).
Article in Histochemistry and cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- The active plant compounds demonstrated positive activity on mouse intestinal organoids as an inflammation model system.In vitro cellular & developmental biology. Animal · 2026Article
- In Vitro Culture of Cryptosporidium spp. Using Stem Cell-Derived Intestinal Epithelial Monolayers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- IPEC-J2 as a cellular model for studying intestinal mucus.Scientific reports · 2025Article
- Development of a Caco-2-based intestinal mucosal model to study intestinal barrier properties and bacteria-mucus interactions.Gut microbes · 2025Article
- Enterocyte Culture on a Hybrid Transwell-Inserted Gut-on-a-Chip for Liquid-Liquid and Air-Liquid Interface Conditions.ACS omega · 2025Article
- Dynamic Mechanical Load as a Trigger for Growth and Proliferation in Porcine Epithelial Cells.Biomolecules · 2025Article
- Article
- Comprehensive evaluation of a pea co-product for piglet nutrition: fibre content, protein digestion, and intestinal barrier function.Journal of animal science · 2025Article
- Influence of Hypoxia on the Airway Epithelium.Physiological research · 2024Review
- A Microphysiological System with an Anaerobic Air-Liquid Interface and Functional Mucus Layer for Coculture of Intestinal Bacteria and Primary Human Colonic Epithelium.Advanced materials interfaces · 2024Article
- Oxygen levels affect oviduct epithelium functions in air-liquid interface culture.Histochemistry and cell biology · 2024Article
- Effect of air-liquid interface on cultured human intestinal epithelial cells.FASEB bioAdvances · 2024Article
- Modeling of culture conditions by culture system, glucose and propionic acid and their impact on metabolic profile in IPEC-J2.PloS one · 2024Article
- In focus in HCB.Histochemistry and cell biology · 2023Article
- Air-liquid interface cultures trigger a metabolic shift in intestinal epithelial cells (IPEC-1).Histochemistry and cell biology · 2023Article
- Cell Cultures at the Air-Liquid Interface and Their Application in Cancer Research.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Intestinal organoids as advanced modeling platforms to study the role of host-microbiome interaction in homeostasis and disease.BMB reports · 2023Review
- From the Dish to the Real World: Modeling Interactions between the Gut and Microorganisms in Gut Organoids by Tailoring the Gut Milieu.International journal of stem cells · 2022Review
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Authors and funding
9 authors.
Funding
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Abstract
The specific function of the epithelium as critical barrier between the intestinal lumen and the organism's internal microenvironment is reflected by permanent maintenance of intercellular junctions and cellular polarity. The intestinal epithelial cells are responsible for absorption of nutritional components, facing mechanical stress and a changing oxygen supplementation via blood stream. Oxygen itself can regulate the barrier and the absorptive function of the epithelium. Therefore, we compared the dish cell culture, the transwell-like membrane culture and the oxygen enriched air-liquid interface (ALI) culture. We demonstrated strong influence of the different culture conditions on morphology and function of intestinal porcine epithelial cell lines in vitro. ALI culture resulted in a significant increase in cell number, epithelial cell layer thickness and expression as well as apical localisation of the microvilli-associated protein villin. Remarkable similarities regarding the morphological parameters were observed between ALI cultures and intestinal epithelial cells in vivo. Furthermore, the functional analysis of protein uptake and degradation by the epithelial cells demonstrated the necessity of sufficient oxygen supply as achieved in ALI cultures. Our study is the first report providing marked evidence that optimised oxygen supply using ALI cultures directly affects the morphological differentiation and functional properties of intestinal epithelial cells in vitro.
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