ArticleEMBO reports2011
The role of trimerization in the osmoregulated betaine transporter BetP.
Article in EMBO reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- They all rock: A systematic comparison of conformational movements in LeuT-fold transporters.Structure (London, England : 1993) · 2024Article
- Structure and mechanism of the osmoregulated choline transporter BetT.Science advances · 2024Article
- Structural and biophysical analysis of aeLife · 2024Article
- Review
- Physicochemical homeostasis in bacteria.FEMS microbiology reviews · 2023Article
- Insights into autoregulation of a membrane protein complex by its cytoplasmic domains.Biophysical journal · 2023Article
- A single-molecule method for measuring fluorophore labeling yields for the study of membrane protein oligomerization in membranes.PloS one · 2023Article
- The Role of the Membrane in Transporter Folding and Activity.Journal of molecular biology · 2021Review
- Trimerization of dopamine transporter triggered by AIM-100 binding: Molecular mechanism and effect of mutations.Neuropharmacology · 2019Article
- Comparison of the functional properties of trimeric and monomeric CaiT of Escherichia coli.Scientific reports · 2019Article
- Interpretation of spectroscopic data using molecular simulations for the secondary active transporter BetP.The Journal of general physiology · 2019Article
- Shared dynamics of LeuT superfamily members and allosteric differentiation by structural irregularities and multimerization.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Human Concentrative Nucleoside Transporter 3 (hCNT3, SLC28A3) Forms a Cyclic Homotrimer.Biochemistry · 2017Article
- The N Terminus Specifies the Switch between Transport Modes of the Human Serotonin Transporter.The Journal of biological chemistry · 2017Article
- Transporter oligomerization: form and function.Biochemical Society transactions · 2016Review
- Lipid-Protein Interactions in the Regulated Betaine Symporter BetP Probed by Infrared Spectroscopy.The Journal of biological chemistry · 2016Article
- Properties of an inward-facing state of LeuT: conformational stability and substrate release.Biophysical journal · 2015Article
- Substrate-bound outward-open state of the betaine transporter BetP provides insights into Na+ coupling.Nature communications · 2014Article
- Structural evidence for functional lipid interactions in the betaine transporter BetP.The EMBO journal · 2013Article
- Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The osmoregulated betaine transporter BetP is a stable trimer. Structural studies have shown that individual protomers can adopt distinct transport conformations, implying a functional role for the trimeric state in transport, although the role of trimerization in regulation is not yet understood. We designed putative monomeric mutants by molecular-dynamics simulations and in silico alanine-scanning mutagenesis. Several mutants including BetP-W101A/T351A were monomeric in detergent as well as in the membrane, as shown by blue native gel electrophoresis, crosslinking and electron microscopy. This monomeric form retains the ability to accumulate betaine, but is no longer regulated by hyperosmotic shock.
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