ArticleThe Journal of biological chemistry2011
Transport activity of the high-affinity monocarboxylate transporter MCT2 is enhanced by extracellular carbonic anhydrase IV but not by intracellular carbonic anhydrase II.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 58 citations in OpenAlex.
- Lactic acid metabolism: gynecological cancer's Achilles' heel.Discover oncology · 2025Review
- GHB toxicokinetics and renal monocarboxylate transporter expression are influenced by the estrus cycle in rats.BMC pharmacology & toxicology · 2023Article
- Local Attraction of Substrates and Co-Substrates Enhances Weak Acid and Base Transmembrane Transport.Biomolecules · 2022Review
- A guide to plasma membrane solute carrier proteins.The FEBS journal · 2021Review
- Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.Advances in experimental medicine and biology · 2021Review
- Cooperative transport mechanism of human monocarboxylate transporter 2.Nature communications · 2020Article
- Review
- Monocarboxylate Transporters (SLC16): Function, Regulation, and Role in Health and Disease.Pharmacological reviews · 2020Review
- CAIX forms a transport metabolon with monocarboxylate transporters in human breast cancer cells.Oncogene · 2020Article
- Astrocytes and neurons communicate via a monocarboxylic acid shuttle.AIMS neuroscience · 2020Review
- pH and male fertility: making sense on pH homeodynamics throughout the male reproductive tract.Cellular and molecular life sciences : CMLS · 2019Review
- Catalytically inactive carbonic anhydrase-related proteins enhance transport of lactate by MCT1.FEBS open bio · 2019Article
- Membrane-anchored carbonic anhydrase IV interacts with monocarboxylate transporters via their chaperones CD147 and GP70.The Journal of biological chemistry · 2019Article
- Lactate in the Regulation of Tumor Microenvironment and Therapeutic Approaches.Frontiers in oncology · 2019Review
- Energy Dynamics in the Brain: Contributions of Astrocytes to Metabolism and pH Homeostasis.Frontiers in neuroscience · 2019Review
- A surface proton antenna in carbonic anhydrase II supports lactate transport in cancer cells.eLife · 2018Article
- The clinicopathological significance of monocarboxylate transporters in testicular germ cell tumors.Oncotarget · 2018Article
- Expression of Carbonic Anhydrase I in Motor Neurons and Alterations in ALS.International journal of molecular sciences · 2016Article
- Monocarboxylate Transporters: Therapeutic Targets and Prognostic Factors in Disease.Clinical pharmacology and therapeutics · 2016Review
- Monocarboxylate transporters in the brain and in cancer.Biochimica et biophysica acta · 2016Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ubiquitous enzyme carbonic anhydrase isoform II (CAII) has been shown to enhance transport activity of the proton-coupled monocarboxylate transporters MCT1 and MCT4 in a non-catalytic manner. In this study, we investigated the role of cytosolic CAII and of the extracellular, membrane-bound CA isoform IV (CAIV) on the lactate transport activity of the high-affinity monocarboxylate transporter MCT2, heterologously expressed in Xenopus oocytes. In contrast to MCT1 and MCT4, transport activity of MCT2 was not altered by CAII. However, coexpression of CAIV with MCT2 resulted in a significant increase in MCT2 transport activity when the transporter was coexpressed with its associated ancillary protein GP70 (embigin). The CAIV-mediated augmentation of MCT2 activity was independent of the catalytic activity of the enzyme, as application of the CA-inhibitor ethoxyzolamide or coexpressing the catalytically inactive mutant CAIV-V165Y did not suppress CAIV-mediated augmentation of MCT2 transport activity. Furthermore, exchange of His-88, mediating an intramolecular H(+)-shuttle in CAIV, to alanine resulted only in a slight decrease in CAIV-mediated augmentation of MCT2 activity. The data suggest that extracellular membrane-bound CAIV, but not cytosolic CAII, augments transport activity of MCT2 in a non-catalytic manner, possibly by facilitating a proton pathway other than His-88.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.