ArticleJournal of virology2011
Assessment of the genetic susceptibility of sheep to scrapie by protein misfolding cyclic amplification and comparison with experimental scrapie transmission studies.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Strain-Dependent Susceptibility to Prion Infection Encoded by Arg171 and Lys176 Sheep Prion Protein Polymorphic Variants.The Journal of infectious diseases · 2025Article
- Development of a methodology for large-scale production of prions for biological and structural studies.Frontiers in molecular biosciences · 2023Article
- Biodiversity and selection for scrapie resistance in sheep: genetic polymorphism in eight breeds of Algeria.Journal of genetics · 2018Article
- Design, implementation, and interpretation of amplification studies for prion detection.Prion · 2018Article
- Estimating chronic wasting disease susceptibility in cervids using real-time quaking-induced conversion.The Journal of general virology · 2017Article
- Region-specific protein misfolding cyclic amplification reproduces brain tropism of prion strains.The Journal of biological chemistry · 2017Article
- Genetic resistance to transmissible spongiform encephalopathies (TSE) in goats.EFSA journal. European Food Safety Authority · 2017Article
- Molecular modeling of prion transmission to humans.Viruses · 2014Article
- Enhanced virulence of sheep-passaged bovine spongiform encephalopathy agent is revealed by decreased polymorphism barriers in prion protein conversion studies.Journal of virology · 2014Article
- In vitro prion protein conversion suggests risk of bighorn sheep (Ovis canadensis) to transmissible spongiform encephalopathies.BMC veterinary research · 2013Article
- In vitro amplification of ovine prions from scrapie-infected sheep from Great Britain reveals distinct patterns of propagation.BMC veterinary research · 2012Article
- Sensitivity of protein misfolding cyclic amplification versus immunohistochemistry in ante-mortem detection of chronic wasting disease.The Journal of general virology · 2012Article
- Fast and ultrasensitive method for quantitating prion infectivity titre.Nature communications · 2012Article
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Authors and funding
12 authors.
Funding
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Abstract
The susceptibility of sheep to scrapie is influenced mainly by the prion protein polymorphisms A136V, R154H, and Q171R/H. Here we analyzed the ability of protein misfolding cyclic amplification (PMCA) to model the genetic susceptibility of sheep to scrapie. For this purpose, we studied the efficiency of brain homogenates from sheep with different PrP genotypes to support PrP(Sc) amplification by PMCA using an ARQ/ARQ scrapie inoculum. The results were then compared with those obtained in vivo using the same sheep breed, genotypes, and scrapie inoculum. Genotypes associated with susceptibility (ARQ/ARQ, ARQ/AHQ, and AHQ/ARH) were able to sustain PrP(Sc) amplification in PMCA reactions, while genotypes associated with resistance to scrapie (ARQ/ARR and ARR/ARR) were unable to support the in vitro conversion. The incubation times of the experimental infection were then compared with the in vitro amplification factors. Linear regression analysis showed that the efficiency of in vitro PrP(Sc) amplification of the different genotypes was indeed inversely proportional to their incubation times. Finally, the rare ARQK₁₇₆/ARQK₁₇₆ genotype, for which no in vivo data are available, was studied by PMCA. No amplification was obtained, suggesting ARQK₁₇₆/ARQK₁₇₆ as an additional genotype associated with resistance, at least to the isolate tested. Our results indicate a direct correlation between the ability of different PrP genotypes to undergo PrP(C)-to-PrP(Sc) conversion by PMCA and their in vivo susceptibility and point to PMCA as an alternative to transmission studies and a potential tool to test the susceptibility of numerous sheep PrP genotypes to a variety of prion sources.
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