ArticleJournal of virology2011
Liposomal nanocontainers as models for viral infection: monitoring viral genomic RNA transfer through lipid membranes.
Article in Journal of virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Open questions in enterovirus uncoating.Journal of virology · 2026Review
- Cryo-EM of human rhinovirus reveals capsid-RNA duplex interactions that provide insights into virus assembly and genome uncoating.Communications biology · 2024Article
- VP2 mediates the release of the feline calicivirus RNA genome by puncturing the endosome membrane of infected cells.Journal of virology · 2024Article
- Structural Studies Reveal that Endosomal Cations Promote Formation of Infectious Coxsackievirus A9 A-Particles, Facilitating RNA and VP4 Release.Journal of virology · 2022Article
- Capsid opening enables genome release of iflaviruses.Science advances · 2021Article
- Nanomedicine strategies to target coronavirus.Nano today · 2020Review
- Liposome-Based Bioassays.Biology · 2020Review
- Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature.PLoS pathogens · 2018Article
- Impairing the function of MLCK, myosin Va or myosin Vb disrupts Rhinovirus B14 replication.Scientific reports · 2017Article
- In vitro RNA release from a human rhinovirus monitored by means of a molecular beacon and chip electrophoresis.Analytical and bioanalytical chemistry · 2016Article
- Viral entry pathways: the example of common cold viruses.Wiener medizinische Wochenschrift (1946) · 2016Review
- Triatoma virus recombinant VP4 protein induces membrane permeability through dynamic pores.Journal of virology · 2015Article
- Capsid protein VP4 of human rhinovirus induces membrane permeability by the formation of a size-selective multimeric pore.PLoS pathogens · 2014Article
- The Rhinovirus subviral a-particle exposes 3'-terminal sequences of its genomic RNA.Journal of virology · 2014Article
- Uncoating of common cold virus is preceded by RNA switching as determined by X-ray and cryo-EM analyses of the subviral A-particle.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Human rhinovirus subviral a particle binds to lipid membranes over a twofold axis of icosahedral symmetry.Journal of virology · 2013Article
- The enterovirus 71 A-particle forms a gateway to allow genome release: a cryoEM study of picornavirus uncoating.PLoS pathogens · 2013Article
- Viral uncoating is directional: exit of the genomic RNA in a common cold virus starts with the poly-(A) tail at the 3'-end.PLoS pathogens · 2013Article
- Productive entry pathways of human rhinoviruses.Advances in virology · 2012Article
- Insights into minor group rhinovirus uncoating: the X-ray structure of the HRV2 empty capsid.PLoS pathogens · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
After uptake into target cells, many nonenveloped viruses undergo conformational changes in the low-pH environment of the endocytic compartment. This results in exposure of amphipathic viral peptides and/or hydrophobic protein domains that are inserted into and either disrupt or perforate the vesicular membranes. The viral nucleic acids thereby gain access to the cytosol and initiate replication. We here demonstrate the in vitro transfer of the single-stranded positive-sense RNA genome of human rhinovirus 2 into liposomes decorated with recombinant very-low-density lipoprotein receptor fragments. Membrane-attached virions were exposed to pH 5.4, mimicking the in vivo pH environment of late endosomes. This triggered the release of the RNA whose arrival in the liposomal lumen was detected via in situ cDNA synthesis by encapsulated reverse transcriptase. Subsequently, cDNA was PCR amplified. At a low ratio between virions and lipids, RNA transfer was positively correlated with virus concentration. However, membranes became leaky at higher virus concentrations, which resulted in decreased cDNA synthesis. In accordance with earlier in vivo data, the RNA passes through the lipid membrane without causing gross damage to vesicles at physiologically relevant virus concentrations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.