ArticleBMC biotechnology2011
RNA interference in marine and freshwater sponges: actin knockdown in Tethya wilhelma and Ephydatia muelleri by ingested dsRNA expressing bacteria.
Article in BMC biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- A methodology for RNAi gene knockdown in the marine annelid Streblospio benedicti by delivering dsRNA.Development (Cambridge, England) · 2026Article
- Pervasive Mitochondrial tRNA Gene Loss in Clade B of Haplosclerid Sponges (Porifera, Demospongiae).Genome biology and evolution · 2025Article
- The Buds of Oscarella lobularis (Porifera, Homoscleromorpha): A New Convenient Model for Sponge Cell and Evolutionary Developmental Biology.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- Outer membrane vesicles from commensal microbes contribute to the sponge Tedania sp. development by regulating the expression level of apoptosis-inducing factor (AIF).Communications biology · 2024Article
- RNAi-directed knockdown in the cnidarian fish blood parasite Sphaerospora molnari.Scientific reports · 2024Article
- The genomes of the aquarium spongesF1000Research · 2024Article
- Freshwater Sponges as a Neglected Reservoir of Bacterial Biodiversity.Microorganisms · 2023Review
- Transfection of Sponge Cells and Intracellular Localization of Cancer-Related MYC, RRAS2, and DRG1 Proteins.Marine drugs · 2023Article
- RNA Interference by Ingested Dsrna-Expressing Bacteria to Study Porphyrin Pigmentation inInternational journal of molecular sciences · 2021Article
- Article
- In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- RNA Interference by Ingested dsRNA-Expressing Bacteria to Study Shell Biosynthesis and Pigmentation in Crassostrea gigas.Marine biotechnology (New York, N.Y.) · 2019Article
- RNA interference may result in unexpected phenotypes in Caenorhabditis elegans.Nucleic acids research · 2019Article
- Article
- Establishment of Transgenesis in the DemospongeGenetics · 2018Article
- Metagenomic sequencing suggests a diversity of RNA interference-like responses to viruses across multicellular eukaryotes.PLoS genetics · 2018Article
- Wnt signaling and polarity in freshwater sponges.BMC evolutionary biology · 2018Article
- The Origin of Animal Multicellularity and Cell Differentiation.Developmental cell · 2017Review
- Trojan Horse Strategy for Non-invasive Interference of Clock Gene in the Oyster Crassostrea gigas.Marine biotechnology (New York, N.Y.) · 2017Article
- Methylation specific targeting of a chromatin remodeling complex from sponges to humans.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
13 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe marine sponge Tethya wilhelma and the freshwater sponge Ephydatia muelleri are emerging model organisms to study evolution, gene regulation, development, and physiology in non-bilaterian animal systems. Thus far, functional methods (i.e., loss or gain of function) for these organisms have not been available.
resultsWe show that soaking developing freshwater sponges in double-stranded RNA and/or feeding marine and freshwater sponges bacteria expressing double-stranded RNA can lead to RNA interference and reduction of targeted transcript levels. These methods, first utilized in C. elegans, have been adapted for the development and feeding style of easily cultured marine and freshwater poriferans. We demonstrate phenotypic changes result from 'knocking down' expression of the actin gene.
conclusionThis technique provides an easy, efficient loss-of-function manipulation for developmental and gene regulatory studies in these important non-bilaterian animals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.