ArticleGenome biology2011
Deep sequencing on genome-wide scale reveals the unique composition and expression patterns of microRNAs in developing pollen of Oryza sativa.
Article in Genome biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 158 citations in OpenAlex.
- Heat stress-induced remodelling of lipid and microRNA networks in Brassica napus germinated pollen and pollen-derived small extracellular vesicles.Journal of experimental botany · 2026Article
- Genetic analysis and molecular mapping of the purple leaf sheath in barley (Hordeum vulgare).The plant genome · 2025Article
- MicroRNA-Mediated Post-Transcriptional Regulation of Enzymes Involved in Herbicide Resistance inPlants (Basel, Switzerland) · 2025Article
- The rice microRNA159-SPOROCYTELESS EAR2 module regulates starch biosynthesis during pollen development and maintains male fertility.The Plant cell · 2024Article
- Genome-Wide Dissection of Selection on microRNA Target Genes Involved in Rice Flower Development.Plants (Basel, Switzerland) · 2024Article
- Transcription Factors and Their Regulatory Roles in the Male Gametophyte Development of Flowering Plants.International journal of molecular sciences · 2024Review
- Cytological observation and RNA-seq analysis reveal novel miRNAs high expression associated with the pollen fertility of neo-tetraploid rice.BMC plant biology · 2023Article
- Involvement of miRNAs in Metabolic Herbicide Resistance to Bispyribac-Sodium inPlants (Basel, Switzerland) · 2022Article
- Integrated miRNA, target mRNA, and metabolome profiling of3 Biotech · 2022Article
- Versatile Roles of Microbes and Small RNAs in Rice and Planthopper Interactions.The plant pathology journal · 2022Review
- Identification of long non-coding RNAs and microRNAs involved in anther development in the tropical Camellia oleifera.BMC genomics · 2022Article
- Deep Sequencing Discovery and Profiling of Known and Novel miRNAs Produced in Response to DNA Damage in Rice.International journal of molecular sciences · 2021Article
- MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice.International journal of molecular sciences · 2021Article
- Article
- MicroRNAs Involved in Regulatory Cytoplasmic Male Sterility by Analysis RNA-seq and Small RNA-seq in Soybean.Frontiers in genetics · 2021Article
- First Steps in the Successful Fertilization of Rice andPlants (Basel, Switzerland) · 2020Review
- Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L. pollen grains and pollen tubes growing in vitro.Protoplasma · 2020Article
- Article
- CircRNA Expression Pattern and ceRNA and miRNA-mRNA Networks Involved in Anther Development in the CMS Line ofInternational journal of molecular sciences · 2019Article
- Small RNA-Seq reveals novel miRNAs shaping the transcriptomic identity of rat brain structures.Life science alliance · 2018Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPollen development in flowering plants requires strict control of the gene expression program and genetic information stability by mechanisms possibly including the miRNA pathway. However, our understanding of the miRNA pathway in pollen development remains limited, and the dynamic profile of miRNAs in developing pollen is unknown.
resultsUsing next-generation sequencing technology, we pyrosequenced small RNA populations from rice uninucleate microspores to tricellular pollen and control sporophytic tissues at the genome-wide level. We identified 292 known miRNAs, including members of all 20 families conserved in plants, and 75 novel miRNAs. Of the 292 known miRNAs, 202 were expressed, with 103 enriched, in developing pollen. More than half of these novel miRNAs displayed pollen-or stage-specific expression. Furthermore, analyzing the 367 miRNAs and their predicted targets indicated that correlation in expression profiles of pollen-enriched known miRNAs and their targets significantly differs from that of sporophyte-enriched known miRNAs and their targets in some functional terms, while novel miRNAs appeared to negatively regulate their targets. Importantly, gene ontology abundance analysis demonstrated chromatin assembly and disassembly was important in the targets of bicellular pollen-expressed novel miRNAs. Principal component analysis revealed pollen of all three stages was discriminated from sporophytes, largely because of the novel and non-conserved known miRNAs.
conclusionsOur study, for the first time, revealed the differences in composition and expression profiles of miRNAs between developing pollen and sporophytes, with novel and non-conserved known miRNAs the main contributors. Our results suggest the important roles of the miRNA pathway in pollen development.
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