ArticleThe Journal of cell biology1990
Myosin II heavy chain null mutant of Dictyostelium exhibits defective intracellular particle movement.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 65 citations in OpenAlex.
- The IplA Ca2+ channel of Dictyostelium discoideum is necessary for chemotaxis mediated through Ca2+, but not through cAMP, and has a fundamental role in natural aggregation.Journal of cell science · 2012Article
- Myosin II is essential for the spatiotemporal organization of traction forces during cell motility.Molecular biology of the cell · 2010Article
- Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions.The Journal of cell biology · 2008Article
- Nucleokinesis in tangentially migrating neurons comprises two alternating phases: forward migration of the Golgi/centrosome associated with centrosome splitting and myosin contraction at the rear.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Distinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts.The Journal of cell biology · 2004Article
- Asymmetric distribution of myosin IIB in migrating endothelial cells is regulated by a rho-dependent kinase and contributes to tail retraction.Molecular biology of the cell · 2003Article
- Reduced protein diffusion rate by cytoskeleton in vegetative and polarized dictyostelium cells.Biophysical journal · 2001Article
- The internal phosphodiesterase RegA is essential for the suppression of lateral pseudopods during Dictyostelium chemotaxis.Molecular biology of the cell · 2000Article
- PAKa, a putative PAK family member, is required for cytokinesis and the regulation of the cytoskeleton in Dictyostelium discoideum cells during chemotaxis.The Journal of cell biology · 1999Article
- Interaptin, an actin-binding protein of the alpha-actinin superfamily in Dictyostelium discoideum, is developmentally and cAMP-regulated and associates with intracellular membrane compartments.The Journal of cell biology · 1998Article
- Ponticulin plays a role in the positional stabilization of pseudopods.The Journal of cell biology · 1995Article
- Organelle-cytoskeletal interactions: actin mutations inhibit meiosis-dependent mitochondrial rearrangement in the budding yeast Saccharomyces cerevisiae.Molecular biology of the cell · 1995Article
- Single-headed myosin II acts as a dominant negative mutation in Dictyostelium.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Actin-dependent mitochondrial motility in mitotic yeast and cell-free systems: identification of a motor activity on the mitochondrial surface.The Journal of cell biology · 1995Article
- Intracellular calcium levels correlate with speed and persistent forward motion in migrating neutrophils.Biophysical journal · 1995Article
- Yeast mitochondria contain ATP-sensitive, reversible actin-binding activity.Molecular biology of the cell · 1994Article
- Gradients in the concentration and assembly of myosin II in living fibroblasts during locomotion and fiber transport.Molecular biology of the cell · 1993Article
- The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility.Molecular biology of the cell · 1993Article
- Dictyostelium mutants lacking the cytoskeletal protein coronin are defective in cytokinesis and cell motility.The Journal of cell biology · 1993Article
- Targeted disruption of the ABP-120 gene leads to cells with altered motility.The Journal of cell biology · 1992Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Both cellular motility and intracellular particle movement are compared between normal Dictyostelium amebae of strain AX4 and amebae of a myosin II heavy chain null mutant, HS2215, using the computer assisted "Dynamic Morphology System." In AX4 cells rapidly translocating in buffer, cytoplasmic expansion is apical and the majority of intracellular particles move anteriorly, towards the site of expansion. When these cells are pulsed with 10(-6) M cAMP, the peak concentration of the natural cAMP wave, cells stop translocating and average particle velocity decreases threefold within 2-4 s after cAMP addition. After 8 s, there is a partial rebound both in cytoplasmic expansion and particle velocity, but in both cases, original apical polarity is lost. In HS2215 cells in buffer, both cellular translocation and average particle velocity are already at the depressed levels observed in normal cells immediately after cAMP addition, and no anterior bias is observed in either the direction of cytoplasmic expansion or the direction of particle movement. The addition of cAMP to myosin-minus cells results in no additional effect. The results demonstrate that myosin II is necessary for (a) the rapid rate of intracellular particle movement, (b) the biased anterior directionality of particle movement, and (c) the rapid inhibition of particle movement by cAMP.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.