Evidence map›Paper›PMID 21678451›Full record

ArticleJournal of medical virology2011

Comparative evaluation of the Seegene Seeplex RV15 and real-time PCR for respiratory virus detection.

David F Bibby, Iain McElarney, Judith Breuer, Duncan A Clark

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of medical virology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to SeeplexJournal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2017
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Immune deficiency: changing spectrum of pathogens.Clinical and experimental immunology · 2015
    Article
  13. Article
  14. Article
  15. Article
  16. Lower respiratory tract virus findings in mechanically ventilated patients with severe community-acquired pneumonia.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2014
    Article
  17. Article
  18. Article
  19. Evaluation of a novel real-time RT-PCR using TOCE technology compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2013
    Article
  20. Clinical and economical impact of multiplex respiratory virus assays.Diagnostic microbiology and infectious disease · 2013
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

David F BibbyDepartment of Virology, Barts & The London NHS Trust, London, UK. david.bibby@bartsandthelondon.nhs.uk
Iain McElarney
Judith Breuer
Duncan A Clark

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Respiratory virus infections contribute substantially both to hospitalizations of young children, and to morbidity in immunocompromised patients such as those with hematological malignancies. Their rapid and accurate diagnosis is essential to patient management. To evaluate the prospective utility of Seeplex® DPO technology in respiratory virus diagnosis, a panel of 99 respiratory samples positive by real-time RT-PCR for one or more viruses was assayed by the Seegene Seeplex® RV12 system. As well as being able to detect all 10 viruses in the real-time RT-PCR system with the exception of enteroviruses, RV12 can also distinguish between the two subgroups of RSV and detect two subgroups of coronaviruses. Seven of the nine viruses in common with the RT-PCR were detected reliably by RV12. Eleven samples RT-PCR-positive for Metapneumovirus and five samples positive for influenza B were not detected by RV12. Seegene developed a second-generation system, RV15, which not only allowed detection of three additional viruses, but also addressed the potential problems with RV12 specificity. To address these concerns, 84 respiratory samples positive for a range of viruses by real-time PCR were assayed with RV15. The results of this evaluation improved significantly upon those seen with RV12. The high throughput capabilities and potential lower technical requirements afforded by the Seeplex® system may offer an alternative to real-time RT-PCR systems.

Indexed as

HumansMolecular Diagnostic TechniquesPolymerase Chain ReactionRespiratory Tract InfectionsSensitivity and SpecificityVirologyVirus DiseasesViruses

Identifiers

PMID21678451
PMCPMC7166927

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.