ArticleBiophysical chemistry2011
Thermodynamic properties and characterization of proteoliposomes rich in microdomains carrying alkaline phosphatase.
Article in Biophysical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Modulation of TNAP activity and apatite formation in biomimetic matrix vesicles studied byBiochimica et biophysica acta. Biomembranes · 2025Article
- Two Receptor Binding Strategy of SARS-CoV-2 Is Mediated by Both the N-Terminal and Receptor-Binding Spike Domain.The journal of physical chemistry. B · 2024Article
- NPP1 and TNAP hydrolyze ATP synergistically during biomineralization.Purinergic signalling · 2023Article
- Mineralization Profile of Annexin A6-Harbouring Proteoliposomes: Shedding Light on the Role of Annexin A6 on Matrix Vesicle-Mediated Mineralization.International journal of molecular sciences · 2022Article
- A new perspective on the function of Tissue Non-Specific Alkaline Phosphatase: from bone mineralization to intra-cellular lipid accumulation.Molecular and cellular biochemistry · 2022Review
- Lipid composition modulates ATP hydrolysis and calcium phosphate mineral propagation by TNAP-harboring proteoliposomes.Archives of biochemistry and biophysics · 2020Article
- Matrix vesicle biomimetics harboring Annexin A5 and alkaline phosphatase bind to the native collagen matrix produced by mineralizing vascular smooth muscle cells.Biochimica et biophysica acta. General subjects · 2020Article
- Localization of Annexin A6 in Matrix Vesicles During Physiological Mineralization.International journal of molecular sciences · 2020Article
- Cholesterol Regulates the Incorporation and Catalytic Activity of Tissue-Nonspecific Alkaline Phosphatase in DPPC Monolayers.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators.Journal of bone and mineral metabolism · 2019Article
- Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?Archives of biochemistry and biophysics · 2019Article
- Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models.Biochimica et biophysica acta. General subjects · 2018Review
- Biophysical aspects of biomineralization.Biophysical reviews · 2017Review
- Topographic analysis by atomic force microscopy of proteoliposomes matrix vesicle mimetics harboring TNAP and AnxA5.Biochimica et biophysica acta. Biomembranes · 2017Article
- Biomedical applications of nanotechnology.Biophysical reviews · 2017Review
- Liposomal systems as carriers for bioactive compounds.Biophysical reviews · 2015Review
- Effects of GPI-anchored TNAP on the dynamic structure of model membranes.Physical chemistry chemical physics : PCCP · 2015Article
- Proteoliposomes with the ability to transport Ca(2+) into the vesicles and hydrolyze phosphosubstrates on their surface.Archives of biochemistry and biophysics · 2015Article
- Proteoliposomes in nanobiotechnology.Biophysical reviews · 2012Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Tissue-nonspecific alkaline phosphatase (TNAP) is associated to the plasma membrane via a GPI-anchor and plays a key role in the biomineralization process. In plasma membranes, most GPI-anchored proteins are associated with "lipid rafts", ordered microdomains enriched in sphingolipids, glycosphingolipids and cholesterol. In order to better understand the role of lipids present in rafts and their interactions with GPI-anchored proteins, the insertion of TNAP into different lipid raft models was studied using dipalmitoylphosphatidylcholine (DPPC), cholesterol (Chol), sphingomyelin (SM) and ganglioside (GM1). Thus, the membrane models studied were binary systems (9:1 molar ratio) containing DPPC:Chol, DPPC:SM and DPPC:GM1, ternary systems (8:1:1 molar ratio) containing DPPC:Chol:SM, DPPC:Chol:GM1 and DPPC:SM:GM1 and finally, a quaternary system (7:1:1:1 molar ratio) containing DPPC:Chol:SM:GM1. Calorimetry analysis of the liposomes and proteoliposomes indicate that lateral phase segregation could be noted only in the presence of cholesterol, with the formation of cholesterol-rich microdomains centered above Tc=41.5°C. The presence of GM1 and SM into DPPC-liposomes influenced mainly ΔH and Δt(1/2) values. The gradual increase in the complexity of the systems decreased the activity of the enzyme incorporated. The presence of the enzyme also fluidifies the systems, as seen by the intense reduction in ∆H values, but do not alter Tc values significantly. Therefore, the study of different microdomains and its biophysical characterization may contribute to the knowledge of the interactions between the lipids present in MVs and its interactions with TNAP.
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