ArticleThe open virology journal2010
Detection of HIV-1 in Saliva: Implications for Case-Identification, Clinical Monitoring and Surveillance for Drug Resistance.
Article in The open virology journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Trial
- Detection of HIV-1 Antibodies in Saliva of Persons Living With HIV Using Blood-Based First Response HIV 1-2.O Card Test.Journal of clinical laboratory analysis · 2025Article
- Evaluating and optimizing Acid-pH and Direct Lysis RNA extraction for SARS-CoV-2 RNA detection in whole saliva.Scientific reports · 2024Article
- Saliva-based methods for SARS-CoV-2 testing in low- and middle-income countries.Bulletin of the World Health Organization · 2022Article
- HIV Transmission Through Premastication.Pediatrics · 2022Article
- Salivary Stress/Immunological Markers in Crohn's Disease and Ulcerative Colitis.International journal of molecular sciences · 2020Review
- Evaluation and diagnostic usefulness of saliva for detection of HIV antibodies: A cross-sectional study.Journal of family medicine and primary care · 2020Article
- Detection of human immunodeficiency virus (HIV) RNA in the sweat of HIV-infected patients.Northern clinics of Istanbul · 2020Article
- Antibody detection by agglutination-PCR (ADAP) enables early diagnosis of HIV infection by oral fluid analysis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Detection of HIV RNA in dried blood spots and oral fluids.AIDS (London, England) · 2017Article
- Saliva and viral infections.Periodontology 2000 · 2016Review
- Does cerumen have a risk for transmission of HIV?European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Noncoding RNAs in human saliva as potential disease biomarkers.Frontiers in genetics · 2015Article
- Quantitative analysis of differentially expressed saliva proteins in human immunodeficiency virus type 1 (HIV-1) infected individuals.Analytica chimica acta · 2013Article
- Evaluation of disease and viral biomarkers as triggers for therapeutic intervention in respiratory mousepox - an animal model of smallpox.Antiviral research · 2012Article
- Human breast milk and antiretrovirals dramatically reduce oral HIV-1 transmission in BLT humanized mice.PLoS pathogens · 2012Article
- Possible transmission of HIV Infection due to human bite.AIDS research and therapy · 2011Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSaliva tests that detect antibodies are used to diagnose HIV infection. The goal of this study was to determine whether saliva could be used for nucleic acid-based tests to measure HIV-1 virus load (VL) and detect drug resistance.
methods69 HIV infected individuals provided 5-10 ml of saliva and blood samples. Viral RNA was isolated from saliva and dried blood spots using the Nuclisens extraction. Saliva VL was measured using a modified Amplicor assay, and genotyping was performed using an in-house RT-PCR/sequencing protocol. Plasma VLs were obtained from concurrently drawn clinical tests.
resultsThirty-six of 47 (77%) plasma viremic patients had measurable saliva HIV-1 RNA. Paired plasma and saliva HIV RNA levels were significantly correlated (Spearman's correlation = .6532, p<.0001), but saliva VL was typically lower. Three of 22 patients with undetectable plasma VL (<50 copies/ml) had detectable saliva HIV RNA. Eleven of 30 patients with undetectable saliva RNA had detectable plasma HIV-1 RNA. Comparison of the protease and reverse transcriptase gene sequences from paired saliva and plasma of 20 patients showed less than 1% difference overall, and few resistance-related amino acid differences
conclusionsMost patients with plasma virus >50 copies/mL had detectable saliva HIV RNA, and the genotypic data was highly concordant between saliva and plasma. In patients with high levels of plasma HIV RNA, saliva might be useful in identifying viremia and evaluating drug resistance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.