ArticleJournal of visualized experiments : JoVE2011
Whole mount preparation of the adult Drosophila ventral nerve cord for giant fiber dye injection.
Article in Journal of visualized experiments : JoVE, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- Frazzled/DCC Regulates Gap Junction Formation at aeNeuro · 2025Article
- Tyrosyl-tRNA synthetase has a noncanonical function in actin bundling.Nature communications · 2023Article
- Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model.BMC biology · 2020Article
- Transcriptional dysregulation by a nucleus-localized aminoacyl-tRNA synthetase associated with Charcot-Marie-Tooth neuropathy.Nature communications · 2019Article
- An ankyrin-binding motif regulates nuclear levels of L1-type neuroglian and expression of the oncogeneThe Journal of biological chemistry · 2018Article
- The Drosophila Gr28bD product is a non-specific cation channel that can be used as a novel thermogenetic tool.Scientific reports · 2018Article
- Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains.Journal of visualized experiments : JoVE · 2017Article
- Fragile X Mental Retardation Protein Restricts Small Dye Iontophoresis Entry into Central Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
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- Lissencephaly-1 dependent axonal retrograde transport of L1-type CAM Neuroglian in the adult drosophila central nervous system.PloS one · 2017Article
- Structure-function analyses of tyrosine phosphatase PTP69D in giant fiber synapse formation of Drosophila.Molecular and cellular neurosciences · 2015Article
- Engrailed alters the specificity of synaptic connections of Drosophila auditory neurons with the giant fiber.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- CMT-associated mutations in glycyl- and tyrosyl-tRNA synthetases exhibit similar pattern of toxicity and share common genetic modifiers in Drosophila.Neurobiology of disease · 2014Article
- Netrin and Frazzled regulate presynaptic gap junctions at a Drosophila giant synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Differential effects of human L1CAM mutations on complementing guidance and synaptic defects in Drosophila melanogaster.PloS one · 2013Article
- Preparation of Drosophila central neurons for in situ patch clamping.Journal of visualized experiments : JoVE · 2012Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
To analyze the axonal and dendritic morphology of neurons, it is essential to obtain accurate labeling of neuronal structures. Preparing well labeled samples with little to no tissue damage enables us to analyze cell morphology and to compare individual samples to each other, hence allowing the identification of mutant anomalies. In the demonstrated dissection method the nervous system remains mostly inside the adult fly. Through a dorsal incision, the abdomen and thorax are opened and most of the internal organs are removed. Only the dorsal side of the ventral nerve cord (VNC) and the cervical connective (CvC) containing the big axons of the giant fibers (GFs) are exposed, while the brain containing the GF cell body and dendrites remains in the intact head. In this preparation most nerves of the VNC should remain attached to their muscles. Following the dissection, the intracellular filling of the giant fiber (GF) with a fluorescent dye is demonstrated. In the CvC the GF axons are located at the dorsal surface and thus can be easily visualized under a microscope with differential interference contrast (DIC) optics. This allows the injection of the GF axons with dye at this site to label the entire GF including the axons and their terminals in the VNC. This method results in reliable and strong staining of the GFs allowing the neurons to be imaged immediately after filling with an epifluorescent microscope. Alternatively, the fluorescent signal can be enhanced using standard immunohistochemistry procedures suitable for high resolution confocal microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.