ArticleProceedings of the National Academy of Sciences of the United States of America2011
Site-specific acetylation mark on an essential chromatin-remodeling complex promotes resistance to replication stress.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Leveraging synthetic genetic array screening to identify therapeutic targets and inhibitors for combatting azole resistance inMicrobiology spectrum · 2025Article
- Epigenetic roles of chromatin remodeling complexes in bone biology and the pathogenesis of bone‑related disease (Review).International journal of molecular medicine · 2025Review
- Accumulation of unacetylatable Snf2p at the INO1 promoter is detrimental to remodeler recycling supply for CUP1 induction.PloS one · 2020Article
- Fungal-induced protein hyperacetylation in maize identified by acetylome profiling.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Nonsense-mediated decay regulates key components of homologous recombination.Nucleic acids research · 2016Article
- Building a KATalogue of acetyllysine targeting and function.Briefings in functional genomics · 2016Review
- The RSC complex localizes to coding sequences to regulate Pol II and histone occupancy.Molecular cell · 2014Article
- Swi/Snf dynamics on stress-responsive genes is governed by competitive bromodomain interactions.Genes & development · 2014Article
- Chromatin remodelling complex RSC promotes base excision repair in chromatin of Saccharomyces cerevisiae.DNA repair · 2014Article
- Article
- A UV-induced genetic network links the RSC complex to nucleotide excision repair and shows dose-dependent rewiring.Cell reports · 2013Article
- High-resolution network biology: connecting sequence with function.Nature reviews. Genetics · 2013Review
- A role for chromatin remodellers in replication of damaged DNA.Nucleic acids research · 2012Article
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12 authors at 2 institutions in 1 country.
Funding
Abstract
Recent work has identified several posttranslational modifications (PTMs) on chromatin-remodeling complexes. Compared with our understanding of histone PTMs, significantly less is known about the functions of PTMs on remodeling complexes, because identification of their specific roles often is hindered by the presence of redundant pathways. Remodels the Structure of Chromatin (RSC) is an essential, multifunctional ATP-dependent chromatin-remodeling enzyme of Saccharomyces cerevisiae that preferentially binds acetylated nucleosomes. RSC is itself acetylated by Gcn5 on lysine 25 (K25) of its Rsc4 subunit, adjacent to two tandem bromodomains. It has been shown that an intramolecular interaction between the acetylation mark and the proximal bromodomain inhibits binding of the second bromodomain to acetylated histone H3 tails. We report here that acetylation does not significantly alter the catalytic activity of RSC or its ability to recognize histone H3-acetylated nucleosomes preferentially in vitro. However, we find that Rsc4 acetylation is crucial for resistance to DNA damage in vivo. Epistatic miniarray profiling of the rsc4-K25R mutant reveals an interaction with mutants in the INO80 complex, a mediator of DNA damage and replication stress tolerance. In the absence of a core INO80 subunit, rsc4-K25R mutants display sensitivity to hydroxyurea and delayed S-phase progression under DNA damage. Thus, Rsc4 helps promote resistance to replication stress, and its single acetylation mark regulates this function. These studies offer an example of acetylation of a chromatin-remodeling enzyme controlling a biological output of the system rather than regulating its core enzymatic properties.
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